       Document 0444
 DOCN  M9610444
 TI    Strategy to detect and identify Bartonella species in routine clinical
       laboratory yields Bartonella henselae from human immunodeficiency
       virus-positive patient and unique Bartonella strain from his cat.
 DT    9601
 AU    Clarridge JE 3rd; Raich TJ; Pirwani D; Simon B; Tsai L;
       Rodriguez-Barradas MC; Regnery R; Zollo A; Jones DC; Rambo C; Laboratory
       Service, Veterans Affairs Medical Center, Houston,; Texas, USA.
 SO    J Clin Microbiol. 1995 Aug;33(8):2107-13. Unique Identifier : AIDSLINE
       MED/96057595
 AB    We wished to develop a cost-effective, rapid strategy to detect and
       identify Bartonella species in the clinical laboratory and to determine
       the prevalence of Bartonella infection in the Houston veteran
       population. Bartonella colonies were identified by colony morphology,
       Gram stain, RapID ANA, repetitive extragenic palindromic-PCR (REP-PCR)
       and whole-cell fatty acid (CFA) analysis, and these methods were
       compared for their usefulness. A new test order for Rochalimaea culture
       (the genus Bartonella was previously known as the genus Rochalimaea) was
       instituted, and in addition, all blood specimens submitted for fungal
       culture (obtained in an isolator tube) were processed for Bartonella
       culture. Over a 16-month period we isolated Bartonella henselae from
       only 0.4% (2 of 533) of total cultures but from 1% (2 of 204) of human
       immunodeficiency virus-positive patients. After sufficient growth,
       identification of the Bartonella isolates to the species level could be
       obtained in 2 days. The REP-PCR allowed discrimination of all known
       species, whereas CFA analysis distinguished all except B. henselae and
       Bartonella quintana. The RapID ANA results failed to differentiate
       between B. henselae and B. quintana, and results for other species
       differed by only one or two tests. Blood obtained from a kitten which
       had been introduced into the household of one patient 2 months before
       the onset of fever yielded a Bartonella strain which was shown to be
       different from the strain from the patient and distinct from other
       Bartonella species by a combination of REP-PCR, CFA, and growth
       characteristics.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Adult  Angiomatosis, Bacillary/*COMPLICATIONS/MICROBIOLOGY/TRANSMISSION
       Animal  AIDS-Related Opportunistic Infections/*MICROBIOLOGY/TRANSMISSION
       Bacteriological Techniques
       Bartonella/CLASSIFICATION/GENETICS/*ISOLATION & PURIF  Bartonella
       henselae/CLASSIFICATION/GENETICS/*ISOLATION & PURIF  Case Report
       Cat-Scratch Disease/COMPLICATIONS/MICROBIOLOGY/TRANSMISSION
       Cats/*MICROBIOLOGY  Disease Reservoirs  Fatty Acids/ANALYSIS  Human
       Male  Middle Age  Polymerase Chain Reaction  Species Specificity
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

