       Document 0441
 DOCN  M9610441
 TI    beta-Endorphin enhances the replication of neurotropic human
       immunodeficiency virus in fetal perivascular microglia.
 DT    9601
 AU    Sundar KS; Kamaraju LS; Dingfelder J; McMahon J; Gollapudi S; Wilson WH;
       Kong LY; Hong JS; Weiss JM; Lee JE; Department of Psychiatry, Duke
       University Medical Center, Durham,; NC 27710, USA.
 SO    J Neuroimmunol. 1995 Aug;61(1):97-104. Unique Identifier : AIDSLINE
       MED/96032339
 AB    The effect of an endogenous opiate, beta-endorphin, on the replication
       of HIV was investigated in brain perivascular microglia. Beta-endorphin
       enhanced the synthesis of p-24 antigen and transactivation of HIV
       promoter. Dialysed culture supernatants of endorphin-treated microglia
       re-activated latent HIV infection. These culture supernatants showed
       elevated levels of interleukin-1 beta, IL-6 and tumor necrosis factor
       alpha. Sub-optimal concentration of beta-endorphin potentiated
       GP-120-induced synthesis of these cytokines. Nalaxone reversed
       beta-endorphin-induced, but not GP-120-induced, cytokine production and
       enhanced HIV replication. These results suggest that endogenous opiates
       may contribute to the progression of AIDS dementia complex.
 DE    beta-Endorphin/*PHARMACOLOGY  Cytokines/BIOSYNTHESIS  Human  HIV/*GROWTH
       & DEVELOPMENT/PATHOGENICITY  HIV Long Terminal Repeat  In Vitro
       Microglia/*MICROBIOLOGY  Naloxone/PHARMACOLOGY  Narcotic
       Antagonists/PHARMACOLOGY  Receptors, Opioid/ANTAGONISTS & INHIB
       Support, U.S. Gov't, P.H.S.  Trans-Activation (Genetics)  Virus Latency
       Virus Replication/*DRUG EFFECTS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

