       Document 0437
 DOCN  M9610437
 TI    Retrospective determination of HIV-1 status by a PCR method on paraffin
       wax embedded sections.
 DT    9601
 AU    Slavik T; Wolfaardt M; van Zyl H; Simson IW; Department of Anatomical
       Pathology, Faculty of Medicine,; University of Pretoria, South Africa.
 SO    J Clin Pathol. 1995 Aug;48(8):733-6. Unique Identifier : AIDSLINE
       MED/96005883
 AB    AIM--To develop a simple but reliable polymerase chain reaction (PCR)
       method to determine the HIV-1 status of patients on formalin fixed,
       paraffin wax embedded lymph node tissue. METHODS--Fifty lymph node
       specimens, 20 from HIV-1 seropositive and 30 from HIV-1 seronegative
       patients, were analysed. Lymph nodes with a variety of disease
       conditions were included in the study. Tissue sections were treated with
       a DNA extraction buffer containing proteinase K and the crude cell
       lysate was used in PCR analysis. Nested primers were used to amplify
       HIV-1 DNA sequences coding for gag, pol and env proteins. PCR products
       were demonstrated by polyacrylamide gel electrophoresis. Results were
       then compared with HIV-1 serology of the patients from whom the tissue
       was obtained. RESULTS--The PCR method yielded a specificity of 100%, a
       sensitivity of 95%, a positive predictive value of 100%, and a negative
       predictive value of 97% when compared with HIV-1 serology. The kappa
       statistic (0.958) showed an excellent agreement between the PCR method
       and serology. Furthermore, HIV-1 DNA was demonstrated in lymph node
       tissue from a serologically unconfirmed acquired immunodeficiency
       syndrome case necropsied in 1982. CONCLUSION--This PCR method is a
       simple and reliable means of retrospectively determining the HIV-1
       status of patients using formalin fixed, paraffin wax embedded lymph
       node tissue.
 DE    Acquired Immunodeficiency Syndrome/VIROLOGY  Human  HIV
       Seropositivity/VIROLOGY  HIV-1/*ISOLATION & PURIF  Lymph Nodes/VIROLOGY
       Polymerase Chain Reaction/*METHODS  Predictive Value of Tests
       Retrospective Studies  Sensitivity and Specificity  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

