       Document 0413
 DOCN  M9610413
 TI    Distinct phenotypes of antigen-selected CD8 T cells emerge at different
       stages of an in vivo immune response.
 DT    9601
 AU    Walker PR; Ohteki T; Lopez JA; MacDonald HR; Maryanski JL; Ludwig
       Institute for Cancer Research, Lausanne Branch, Epalinges,; Switzerland.
 SO    J Immunol. 1995 Oct 1;155(7):3443-52. Unique Identifier : AIDSLINE
       MED/96015989
 AB    We have previously described a unique system for identifying Ag-selected
       CD8 T cells during an in vivo response in normal mice. In this system,
       lymphocytes isolated from DBA/2 mice injected i.p. with HLA-CW3
       transfected syngeneic (H-2d) P815 cells show a remarkable expansion of
       CD8 cells that utilize TCR expressing the V beta 10 gene segment and
       additional structural features characteristic of Kd-restricted
       CW3-specific CTL clones. We have now taken advantage of this system to
       characterize the surface phenotype of CD8 cells selected by Ag in vivo.
       We observed several distinct phenotypes at different stages of the
       response. At the peak of the response, Ag-selected cells were low in
       CD62L and CD45RB expression but displayed high levels of CD44. In
       addition, there was a partial down-regulation of CD8 and TCR. Cells of
       this phenotype were present in lymphoid tissues for several mo after
       immunization. Much later in the response, Ag-selected cells expressed
       higher levels of CD8 and TCR. Moreover, a distinct subset of these
       long-term immune cells emerged that now expressed CD62L and CD45RB.
       Analysis of CD8 cells from different tissues also revealed certain
       differences, particularly in TCR and co-receptor levels from
       liver-derived cells compared with circulating cells at the peak of the
       response. Our findings suggest that the function of Ag-selected CD8
       cells may be regulated over time and according to location by subtle
       changes in cell-surface phenotype.
 DE    Animal  Antigen Presentation  CD8-Positive T-Lymphocytes/*IMMUNOLOGY
       Female  *Immunity, Cellular  Immunophenotyping  Lymphoid
       Tissue/*IMMUNOLOGY  Mice  Organ Specificity  T-Lymphocyte Subsets
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

