       Document 0410
 DOCN  M9610410
 TI    Molecular characterization of fusion regulatory protein-1 (FRP-1) that
       induces multinucleated giant cell formation of monocytes and HIV
       gp160-mediated cell fusion. FRP-1 and 4F2/CD98 are identical molecules.
 DT    9601
 AU    Ohgimoto S; Tabata N; Suga S; Nishio M; Ohta H; Tsurudome M; Komada H;
       Kawano M; Watanabe N; Ito Y; Department of Microbiology, Mie University
       School of Medicine,; Japan.
 SO    J Immunol. 1995 Oct 1;155(7):3585-92. Unique Identifier : AIDSLINE
       MED/96016007
 AB    Fusion regulatory protein (FRP)-1 regulates virus-mediated cell fusion
       and fusion of monocytes. Eleven of fifteen N-terminal amino acids of
       FRP-1 were the same as the amino acid sequence of 4F2/CD98 heavy chain.
       FRP-1 molecules were detected in Con A- or IL-2-stimulated lymphocytes,
       while FRP-1 was rare on resting lymphocytes. These properties of FRP-1
       are similar to those of 4F2/CD98. Treatment of monocytes with
       anti-4F2/CD98 mAbs resulted in cell fusion, and other mAbs directed
       against 4F2/CD98 induced formation of multinucleated giant cells of
       Cd+U2ME-7 cells, a CD4+U937 cell line transfected with the HIV gp160
       gene. Both anti-4F2/CD98 and anti-FRP-1 mAbs reacted with murine L929
       cells expressing human 4F2/CD98 transiently or constitutively. When
       Newcastle disease virus (NDV)-infected L929 cells expressing human
       FRP-1/CD98 were incubated with mAb 4-5-1, an anti-FRP-1 mAb,
       multinucleated giant cells were induced; thus, FRP-1/CD98 molecules
       expressed in L929 cells are functional for fusion regulatory activity.
 DE    Amino Acid Sequence  Antigens, CD/*CHEMISTRY/IMMUNOLOGY  Cell
       Fusion/DRUG EFFECTS  Gene Products, env/*PHARMACOLOGY  Giant
       Cells/CYTOLOGY/*IMMUNOLOGY  Hela Cells  Human  Leukocytes,
       Mononuclear/CHEMISTRY/*CYTOLOGY/IMMUNOLOGY  Molecular Sequence Data
       Monocytes/CHEMISTRY/*CYTOLOGY/IMMUNOLOGY  Protein
       Precursors/*PHARMACOLOGY  Support, Non-U.S. Gov't  Viral Fusion
       Proteins/*CHEMISTRY/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

