       Document 0408
 DOCN  M9610408
 TI    Laser light suicide of proliferating virus-specific CD8+ T cells in an
       in vivo response.
 DT    9601
 AU    Tripp RA; Lahti JM; Doherty PC; Department of Immunology, St. Jude
       Children's Research Hospital,; Memphis, TN 38105, USA.
 SO    J Immunol. 1995 Oct 15;155(8):3719-21. Unique Identifier : AIDSLINE
       MED/96003407
 AB    Virus-specific CD8+ CTL precursor (CTLp) frequencies are a function of
       rates of clonal expansion and loss, both of which are difficult to
       assess in vivo. Proliferating T cells incorporate the nucleoside
       analogue 5-bromo-2'-deoxyuridine (BrdU), making them sensitive to
       light-induced apoptotic cell death (suicide). Mice were infected with an
       influenza A virus, then given 50 mg of BrdU 24 h before sampling.
       Exposure of freshly isolated, CD8-labeled T cells to the laser beam of
       the flow cytometer resulted in the elimination of > 90% of the
       responding CTLp. The effect was obvious for the regional lymph node from
       day 6 to day 12 after priming, indicating continued cycling over a 7-day
       interval. Thus, as CTLp frequencies remain fairly constant from 7 to 30
       days after infection, the persistent increase (> 30x) in numbers must be
       accompanied by a very substantial loss of virus-specific CD8+ T cells.
 DE    Animal  Bromodeoxyuridine  Cell Death/*IMMUNOLOGY  Cell
       Division/IMMUNOLOGY  CD8-Positive T-Lymphocytes/*IMMUNOLOGY/VIROLOGY
       Female  Immunologic Memory  Lasers  Light  Lymphocyte
       Transformation/IMMUNOLOGY  Mice  Mice, Inbred C57BL  Orthomyxoviruses
       Type A/*IMMUNOLOGY  Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

