       Document 0400
 DOCN  M9610400
 TI    Cytokine patterns during progression to AIDS in children with perinatal
       HIV infection.
 DT    9601
 AU    Hyjek E; Lischner HW; Hyslop T; Bartkowiak J; Kubin M; Trinchieri G;
       Kozbor D; Department of Microbiology and Immunology, Thomas Jefferson;
       University, Philadelphia, PA 19107, USA.
 SO    J Immunol. 1995 Oct 15;155(8):4060-71. Unique Identifier : AIDSLINE
       MED/96003450
 AB    Patterns of cytokine expression were analyzed in polyclonal and
       antigenic responses in children with perinatal HIV infection. Responses
       of PBL to PMA and A23187 calcium ionophore studied in patients in
       different stages of HIV infection revealed reduced levels of IL-2 in
       HIV-infected children beginning before 6 mo of age, and age-dependent
       increases in expression of IL-4, IL-10, and IFN-gamma. The levels of
       IL-4, IL-10, and IFN-gamma expression did not differ significantly
       between HIV-infected and age-matched uninfected children of
       HIV-seropositive mothers, except for a small reduction in HIV-infected
       children in late stages of infection. Responses to PHA, HLA
       alloantigens, HIV envelope peptides T1 and P18, and tetanus toxoid were
       studied in PBMC derived from asymptomatic and mildly symptomatic
       HIV-infected children. IL-2, IFN-gamma, IL-4, and IL-5 expression was
       detected in PHA-stimulated PBMC from all analyzed patients. HIV-infected
       children who failed to respond to HLA alloantigens, tetanus toxoid, or
       the envelope peptides had lower numbers of CD4+ cells and expressed, on
       PHA stimulation, higher levels of IL-4 and IL-5 and lower levels of IL-2
       and IFN-gamma than patients who responded to the antigenic stimulation.
       Results of these analyses suggest that cytokine expression in
       HIV-infected children depends on the character of the stimuli as well as
       the phenotype of PBMC, and indicate possible prevalence of Th2
       Ag-specific responses during the progression of HIV-induced
       immunodeficiency.
 DE    Acquired Immunodeficiency Syndrome/DRUG THERAPY/ETIOLOGY/  *IMMUNOLOGY
       Cytokines/ANALYSIS/*METABOLISM  Disease Progression  Female  Human  HIV
       Infections/*IMMUNOLOGY  Infant  Infant, Newborn  Ionomycin/PHARMACOLOGY
       Lymphocyte Transformation  Pregnancy  Pregnancy Complications,
       Infectious/*IMMUNOLOGY  Prospective Studies  RNA, Messenger/ANALYSIS
       Support, U.S. Gov't, P.H.S.  Tetradecanoylphorbol Acetate/PHARMACOLOGY
       Th1 Cells/*METABOLISM  Th2 Cells/*METABOLISM  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

