       Document 0385
 DOCN  M9610385
 TI    High levels of human peripheral blood mononuclear cell engraftment and
       enhanced susceptibility to human immunodeficiency virus type 1 infection
       in NOD/LtSz-scid/scid mice.
 DT    9601
 AU    Hesselton RM; Greiner DL; Mordes JP; Rajan TV; Sullivan JL; Shultz LD;
       Department of Pediatrics, University of Massachusetts Medical; School,
       Worcester, USA.
 SO    J Infect Dis. 1995 Oct;172(4):974-82. Unique Identifier : AIDSLINE
       MED/96029363
 AB    Inbred C.B-17-scid/scid mice accept human peripheral blood mononuclear
       cell (PBMC) xenografts and are susceptible to human immunodeficiency
       virus type 1 (HIV-1) infection, but low levels of PBMC engraftment
       impede use of this system in HIV research. This report describes the
       effect of host strain background on human PBMC engraftment and HIV
       infectivity in scid mice. Back-crossing the scid mutation to the NOD/Lt
       strain (designated NOD/LtSz-scid/scid) increased the percentage of
       engrafted human PBMC in recipient spleens by 5- to 10-fold compared with
       that in C.B-17-scid/scid stock. Four weeks after human PBMC-injected
       mice were infected with HIV-1, 79% of NOD/LtSz-scid/scid spleens
       harbored replicating virus compared with only 39% of spleens in
       C.B-17-scid/scid mice. The NOD/LtSz-scid/scid mouse may provide a useful
       small animal model for studies of HIV-1.
 DE    Animal  Comparative Study  Disease Susceptibility  DNA,
       Viral/GENETICS/ISOLATION & PURIF  Flow Cytometry  Human  *HIV Infections
       HIV-1/*GROWTH & DEVELOPMENT  Leukocytes, Mononuclear/*TRANSPLANTATION
       Lymph Nodes/CYTOLOGY  Lymphocyte Depletion  Mice  Mice, Inbred NOD
       Mice, SCID  Polymerase Chain Reaction  Species Specificity
       Spleen/CYTOLOGY  Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       Thymus Gland/CYTOLOGY  *Transplantation, Heterologous  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

