       Document 0384
 DOCN  M9610384
 TI    Characterization of Streptococcus pneumoniae from human immunodeficiency
       virus--seropositive patients with acute and recurrent pneumonia.
 DT    9601
 AU    Jordens JZ; Paul J; Bates J; Beaumont C; Kimari J; Gilks C; Public
       Health Laboratory, John Radcliffe Hospital, Headington,; Oxford, United
       Kingdom.
 SO    J Infect Dis. 1995 Oct;172(4):983-7. Unique Identifier : AIDSLINE
       MED/96029364
 AB    Thirty-two isolates of clinically significant Streptococcus pneumoniae
       from 11 human immunodeficiency virus (HIV)-seropositive patients with
       single or multiple episodes of pneumonia were characterized by
       antibiotic susceptibility testing, serotyping, ribotyping, and
       repetitive extragenic palindromic polymerase chain reaction (REP-PCR).
       The isolates comprised 10 serotypes, 12 ribotyping patterns, and 12
       REP-PCR patterns. There was close but not absolute correlation between
       techniques. By combining these characterization methods, 14 strains were
       identified. Five strains were found in > 1 patient, suggesting their
       frequent occurrence in this population. Two isolates of different
       serotype from 1 patient were highly related by ribotyping and REP-PCR,
       suggesting possible in vivo serotype change. Acute infection was
       associated with single strains or coinfection by distinct strains.
       Recurrent pneumonia was identified as relapse with the same strain or
       reinfection with new strains. The molecular characterization of
       pneumonococci from HIV-seropositive persons refines our understanding of
       pneumonococcal infection in these patients.
 DE    Acute Disease  Adult  Bacterial Typing Techniques  DNA,
       Ribosomal/GENETICS  Human  HIV Seropositivity/*COMPLICATIONS
       Kenya/EPIDEMIOLOGY  Microbial Sensitivity Tests  Pneumonia,
       Lobar/*COMPLICATIONS/*EPIDEMIOLOGY  Polymerase Chain Reaction
       Recurrence  Serotyping  Streptococcus
       pneumoniae/*CLASSIFICATION/GENETICS  Support, Non-U.S. Gov't  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

