       Document 0380
 DOCN  M9610380
 TI    Microsporidium: modified technique for light microscopic diagnosis.
 DT    9601
 AU    Punpoowong B; Pitisuttithum P; Chindanond D; Phiboonnakit D;
       Leelasuphasri S; Department of Tropical Pathology, Hospital for Tropical
       Diseases,; Faculty of Tropical Medicine, Mahidol University, Bangkok,;
       Thailand.
 SO    J Med Assoc Thai. 1995 May;78(5):251-4. Unique Identifier : AIDSLINE
       MED/96007040
 AB    Microsporiodosis caused by Enterocytozoon bieneusi is one of the
       opportunistic infections in HIV positive patients with chronic diarrhea.
       The organism is difficult to diagnose because of its small size,
       previously the diagnosis of this infection relied on identification of
       the organism under electron microscope. Until recently, the spores of
       this organism in stool specimens could be seen under light microscope by
       using various staining techniques. In this study, the modified trichrome
       staining technique was used to identify microsporidia spores with
       characteristic red belt-like stripes. Less time and less reagent are
       required by this modified technique than the conventional method.
 DE    Animal  AIDS-Related Opportunistic Infections/*DIAGNOSIS
       Feces/PARASITOLOGY  Human  Microspora Infections/*DIAGNOSIS
       Microsporida/*ISOLATION & PURIF  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

