       Document 0367
 DOCN  M9610367
 TI    Construction of human immunodeficiency virus 1/simian immunodeficiency
       virus strain mac chimeric viruses having vpr and/or nef of different
       parental origins and their in vitro and in vivo replication.
 DT    9601
 AU    Kuwata T; Igarashi T; Ido E; Jin M; Mizuno A; Chen J; Hayami M;
       Institute for Virus Research, Kyoto University, Japan.
 SO    J Gen Virol. 1995 Sep;76 ( Pt 9):2181-91. Unique Identifier : AIDSLINE
       MED/96005039
 AB    We constructed a series of human immunodeficiency virus 1 (HIV-1)/simian
       immunodeficiency virus strain mac (SIVmac) chimeric viruses having vpr
       and/or nef genes of either HIV-1 or SIVmac based on a chimeric virus
       with LTRs, gag, pol, vif and vpx derived from SIVmac and tar, rev, vpu
       and env from HIV-1. All of the chimeric viruses replicated in human and
       macaque peripheral blood mononuclear cells (PBMCs) and in several CD4+
       human cell lines, though their growth potentials were slightly different
       depending on whether vpr and nef were from HIV-1 or SIVmac, or were
       defective. The presence of nef accelerated replication in all the cells
       used and the replication of each chimera appeared to reflect that of the
       parental virus from which nef was derived. The presence of vpr had no
       clear effect in human and monkey PBMCs, but the replication of each
       chimera was influenced by the origin of vpr in H9 and A3.01 cells.
       NM-3rN, which carries HIV-1 vpr and SIVmac nef, was inoculated
       intravenously into three rhesus monkeys, three cynomolgus monkeys and
       two pig-tailed monkeys. From 2 to 14 weeks after inoculation, viruses
       were consistently re-isolated from all the monkeys and virus loads were
       as high as that of SIVmac reported previously. The results indicate that
       infection with NM-3rN is more efficient than any of our previous
       chimeric viruses and suggest that NM-3rN, having HIV-1 Env, will be a
       useful challenge virus for evaluating AIDS vaccines based on HIV-1 Env
       in macaque monkeys instead of chimpanzees.
 DE    Animal  Base Sequence  Cell Line  Cloning, Molecular  DNA, Viral  Female
       Gene Products, nef/BIOSYNTHESIS/*GENETICS  Gene Products,
       vpr/BIOSYNTHESIS/*GENETICS  Human  HIV Infections/VIROLOGY
       HIV-1/*GENETICS/PHYSIOLOGY  Macaca  Male  Molecular Sequence Data
       Reassortant Viruses/*GENETICS/METABOLISM  Simian Acquired
       Immunodeficiency Syndrome/VIROLOGY  Support, Non-U.S. Gov't
       SIV/*GENETICS/PHYSIOLOGY  Viral Proteins/BIOSYNTHESIS  *Virus
       Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

