       Document 0366
 DOCN  M9610366
 TI    Temporal patterns of feline immunodeficiency virus transcripts in
       peripheral blood cells during the latent stage of infection.
 DT    9601
 AU    Tomonaga K; Inoshima Y; Ikeda Y; Mikami T; Department of Veterinary
       Microbiology, Faculty of Agriculture,; University of Tokyo, Japan.
 SO    J Gen Virol. 1995 Sep;76 ( Pt 9):2193-204. Unique Identifier : AIDSLINE
       MED/96005040
 AB    We have investigated the in vivo state of feline immunodeficiency virus
       (FIV) transcription in peripheral blood mononuclear cells (PBMC) of
       chronically FIV-infected, asymptomatic cats. FIV was detected in a high
       percentage of PBMC but not in the plasma of these cats. By quantitative
       reverse transcription-PCR (RT-PCR) analysis. FIV transcriptional status
       in the PBMC was characterized by extremely low or undetectable levels of
       unspliced or singly spliced mRNAs and predominantly multiply spliced
       mRNAs. Upon stimulation in vitro, however, the larger mRNA species and
       infectious virus production were rapidly induced in the PBMC.
       Furthermore, we demonstrated that viral production was induced in
       association with differential increases in the levels of each multiply
       spliced mRNA coding for FIV regulatory proteins. From these results, we
       suggest that replication of FIV is blocked at an early stage of gene
       expression in vivo, as described in asymptomatic human immunodeficiency
       virus (HIV) infected patients, and that FIV infection in cats may be a
       useful model for clinical latency of HIV infection in man. Moreover, we
       propose that the replication of FIV in vivo may be controlled by the
       differential expression of each multiply spliced mRNA.
 DE    Animal  Base Sequence  Cats  Chronic Disease  DNA Primers  DNA, Viral
       Feline Acquired Immunodeficiency Syndrome/BLOOD/*VIROLOGY
       Immunodeficiency Virus, Feline/*GENETICS  Leukocytes,
       Mononuclear/*VIROLOGY  Molecular Sequence Data
       Phytohemagglutinins/PHARMACOLOGY  Polymerase Chain Reaction  RNA
       Splicing  RNA, Messenger/BLOOD  RNA, Viral/*BLOOD  Support, Non-U.S.
       Gov't  Transcription, Genetic/DRUG EFFECTS  Virus Activation  Virus
       Latency  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

