       Document 0365
 DOCN  M9610365
 TI    Paralysis caused by acute myelitis in Theiler's murine encephalomyelitis
       virus strain GD VII infection is induced by CD4+ lymphocytes
       infiltrating the spinal cord.
 DT    9601
 AU    Kohanawa M; Asano M; Min Y; Minagawa T; Nakane A; Department of
       Microbiology, Hokkaido University School of; Medicine, Sapporo, Japan.
 SO    J Gen Virol. 1995 Sep;76 ( Pt 9):2251-6. Unique Identifier : AIDSLINE
       MED/96005046
 AB    Intravenous infection by Theiler's murine encephalomyelitis virus strain
       GD VII causes acute encephalomyelitis and paralysis in infected mice.
       However, nude mice and cyclophosphamide-treated ddY mice did not show
       paralysis when they were able to survive until day 20 post-infection
       (p.i.). Of ddY mice infected with 5 x 10(7) p.f.u./mouse, 70-80% showed
       symptoms of paralysis on day 20 p.i. The viral titres in the brain and
       spinal cord in infected mice were not significantly different between
       paralytic and non-paralytic mice. In all of the mice infected with the
       virus, CD4+ lymphocytes and CD8+ lymphocytes had infiltrated the brain
       on days 10, 12, 14 and 20 p.i. as demonstrated by flow cytometric
       analysis. In contrast, few T lymphocytes infiltrated the spinal cord in
       the non-paralytic mice. Administration of an anti-CD4 monoclonal
       antibody (MAb) or anti-T cell receptor-alpha beta MAb on day 6 p.i.
       inhibited paralysis until day 20 p.i., though 20% of the MAb-treated
       mice and 80% of the control mice showed paralysis. Administration of
       anti-CD8 MAb was not effective in the suppression of paralysis. The MAb
       treatment did not significantly augment viral replication in the spinal
       cord, although the viral titres in the brain of the MAb-treated mice
       increased significantly. After the transfer of spleen cells from
       infected C3H mice, the recipient mice infected with a small amount of
       the virus showed paralysis, though uninfected mice did not. This
       transfer could be blocked by CD4+ lymphocyte depletion of the donor
       mice. These results indicate that paralysis caused by acute myelitis in
       Theiler's virus strain GD VII infection is induced by CD4+ lymphocytes
       infiltrating the spinal cord.
 DE    Acute Disease  Animal  Brain/IMMUNOLOGY/VIROLOGY  Cell Line
       Cyclophosphamide/PHARMACOLOGY  CD4-Positive T-Lymphocytes/*IMMUNOLOGY
       CD8-Positive T-Lymphocytes/IMMUNOLOGY  Female  Flow Cytometry
       Immunosuppressive Agents/PHARMACOLOGY  Immunotherapy, Adoptive
       Lymphocyte Depletion  Male  Mice  Mice, Inbred C3H  Mice, Inbred ICR
       Mice, Nude  Paralysis/IMMUNOLOGY/*VIROLOGY
       Poliomyelitis/*IMMUNOLOGY/VIROLOGY  Polioviruses,
       Murine/*IMMUNOLOGY/PHYSIOLOGY  Receptors, Antigen, T-Cell,
       alpha-beta/IMMUNOLOGY  Spinal Cord/*IMMUNOLOGY  Spleen/CYTOLOGY  Virus
       Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

