       Document 0362
 DOCN  M9610362
 TI    Reverse transcriptase mutations in sequential HIV-1 isolates in a
       patient with AIDS.
 DT    9601
 AU    Gurusinghe AD; Land SA; Birch C; McGavin C; Hooker DJ; Tachedjian G;
       Doherty R; Deacon NJ; AIDS Molecular Biology Laboratory, Macfarlane
       Burnet Centre for; Medical Research, Fairfield, Victoria, Australia.
 SO    J Med Virol. 1995 Jul;46(3):238-43. Unique Identifier : AIDSLINE
       GENBANK/Z31374
 AB    Sequential human immunodeficiency virus type 1 (HIV-1) isolates were
       obtained over a 29-month period from a person before, during, and after
       AZT therapy. DNA sequence analysis of polymerase chain-amplified
       reverse-transcriptase gene showed a gradual accumulation of mutations to
       peak resistance (IC50 2.13 microM AZT) in association with mutations at
       codons 44, 210, and 369, as well as at 41, 67, 70, and 215. Eight months
       after cessation of AZT therapy, when an HIV-1 isolate from the patient
       was again sensitive to AZT, these mutations had all returned to the
       pretherapy sequence.
 DE    Acquired Immunodeficiency Syndrome/DRUG THERAPY/*VIROLOGY  Adult  Amino
       Acid Sequence  Base Sequence  Case Report  Cells, Cultured  DNA, Viral
       Drug Resistance, Microbial  Female  Follow-Up Studies  Genes, Viral
       HIV-1/DRUG EFFECTS/*ENZYMOLOGY/GENETICS/ISOLATION & PURIF  Human
       Microbial Sensitivity Tests  Molecular Sequence Data  *Mutation
       RNA-Directed DNA Polymerase/*GENETICS  Support, Non-U.S. Gov't
       Zidovudine/*THERAPEUTIC USE  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

