       Document 0349
 DOCN  M9610349
 TI    Detection of herpes simplex virus DNA in spontaneous abortions from
       HIV-positive women using non-isotopic in situ hybridization.
 DT    9601
 AU    Wright CA; Haffajee Z; van Iddekinge B; Cooper K; Department of
       Anatomical Pathology School of Pathology, South; African Institute for
       Medical Research, Johannesburg.
 SO    J Pathol. 1995 Aug;176(4):399-402. Unique Identifier : AIDSLINE
       MED/96030726
 AB    The purpose of this study was to determine the prevalence of Herpes
       simplex virus (HSV) endometritis in spontaneous abortions in
       HIV-positive women using non-isotopic in situ hybridization (NISH).
       Post-abortal endometrial curettings from 18 HIV-positive women were
       investigated for the presence of HSV-1 and HSV-2 DNA with NISH. In
       addition, 18 unselected post-abortal endometrial curettings in
       HIV-negative women were used as controls, together with samples of
       normal proliferative and secretory endometrium. Thirteen of the 18
       specimens (72 per cent) from the HIV-positive study group demonstrated
       the presence of HSV DNA, while 2 of the 18 HIV-negative group (11 per
       cent) showed a positive signal. Although the prevalence of HSV
       endometritis in the HIV-positive group was significantly higher than in
       the HIV-negative group (P < 0.05), a causal role for the virus in
       inducing the abortion remains to be determined. In addition, the
       significance of HSV endometritis with regard to the clinical management
       of HIV-positive patients is as yet uncertain.
 DE    Abortion/*VIROLOGY  Adolescence  Adult  DNA, Viral/*ANALYSIS
       Endometritis/VIROLOGY  Female  Herpesviridae
       Infections/*COMPLICATIONS/PATHOLOGY  Herpesvirus 1, Human/ISOLATION &
       PURIF  Herpesvirus 2, Human/ISOLATION & PURIF  Human  HIV
       Seropositivity/*COMPLICATIONS  In Situ Hybridization  Pregnancy
       Simplexvirus/*ISOLATION & PURIF  Support, Non-U.S. Gov't  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

