       Document 0292
 DOCN  M9610292
 TI    Characterization of a HTLV-I-infected cell line derived from a patient
       with adult T-cell leukemia with stable co-expression of CD4 and CD8.
 DT    9601
 AU    Rowe T; Dezzutti C; Guenthner PC; Lam L; Hodge T; Lairmore MD; Lal RB;
       Folks TM; Retrovirus Diseases Branch, Centers for Disease Control and;
       Prevention, Atlanta, GA 30333, USA.
 SO    Leuk Res. 1995 Sep;19(9):621-8. Unique Identifier : AIDSLINE
       MED/96033078
 AB    A long-term T-cell line, termed SP+, was developed from a human T-cell
       leukemia virus type I (HTLV-I)-infected patient with adult T-cell
       leukemia that is dependent on exogenous IL-2 for growth. The SP+
       expresses a full complimentation of HTLV-I-specific viral proteins, and
       contains replication competent viral particles. Restriction enzyme
       digestion followed by Southern blot analysis demonstrated the presence
       of a single integrated proviral copy and limiting dilution analysis
       confirmed the clonality of the cell line. Interestingly, phenotypically,
       the SP+ cell line is CD2+, CD3+ and coexpresses CD4 and CD8, yet lacks
       TCR alpha beta and TCR tau delta expression. Further ontogenetic
       characterization of the SP+ cell line demonstrated the lack of thymic
       T-cell precursor markers, including absence of cell surface expression
       of CD1, intracellular thymic terminal deoxynucleotidyl transferase (TdT)
       enzyme, as well as message expression for V(D)J recombinase activating
       gene-1 (RAG-1). Furthermore, the SP+ cell did express the message for
       the CD3 delta chain. Taken together, these data suggest that the SP+
       cell line resulted from HTLV-I infection of a mature CD4+/CDB+
       lymphocyte. This cell line can be potentially useful as a model, both
       for regulation of cellular functions by HTLV-I and for immunologic
       functions of mature dual CD4/CD8 positive T-cells.
 DE    Antigens, CD4/*METABOLISM  Antigens, CD8/*METABOLISM  Antigens,
       Viral/ANALYSIS  Base Sequence  DNA Primers/CHEMISTRY  Female  Gene
       Expression  Human  HIV/GROWTH & DEVELOPMENT  HTLV-I/GROWTH & DEVELOPMENT
       Immunophenotyping  Infant  Karyotyping  Leukemia,
       T-Cell/MICROBIOLOGY/*PATHOLOGY  Middle Age  Molecular Sequence Data
       Proteins/GENETICS  RNA, Messenger/GENETICS  RNA, Neoplasm/GENETICS
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Thymus
       Gland/CYTOLOGY  Tumor Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

