       Document 0235
 DOCN  M9610235
 TI    Protection against mycoplasma infection using expression-library
       immunization.
 DT    9601
 AU    Barry MA; Lai WC; Johnston SA; Department of Medicine, University of
       Texas Southwestern Medical; Center, Dallas 75235-8573, USA.
 SO    Nature. 1995 Oct 19;377(6550):632-5. Unique Identifier : AIDSLINE
       MED/96026302
 AB    As is evident from the human immunodeficiency virus epidemic, there is
       no systematic method for producing a vaccine. Genetic immunization is a
       new approach to vaccine production that has many of the advantages of
       live/attenuated pathogens but no risk of infection. It involves
       introducing DNA encoding a pathogen protein into host cells and has
       shown promise in several disease models. Here we describe a new method
       for vaccine development, expression-library immunization, which makes
       use of the technique of genetic immunization and the fact that all the
       antigens of a pathogen are encoded in its DNA. An expression library of
       pathogen DNA is used to immunize a host thereby producing the effects of
       antigen presentation of a live vaccine without the risk. We show that
       even partial expression libraries made from the DNA of Mycoplasma
       pulmonis, a natural pathogen in rodents, provide protection against
       challenge from the pathogen. Expression library immunization may prove
       to be a general method for vaccination against any pathogen.
 DE    Animal  Antibodies, Bacterial/BIOSYNTHESIS  Base Sequence  DNA,
       Bacterial/*ADMINISTRATION & DOSAGE/GENETICS/IMMUNOLOGY  DNA,
       Recombinant/*ADMINISTRATION & DOSAGE/GENETICS/IMMUNOLOGY  Gene Library
       Human  Lung/IMMUNOLOGY/PATHOLOGY  Lung
       Diseases/IMMUNOLOGY/MICROBIOLOGY/PATHOLOGY/PREVENTION &  CONTROL  Mice
       Molecular Sequence Data  Mycoplasma Infections/IMMUNOLOGY/*PREVENTION &
       CONTROL  Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

