       Document 0213
 DOCN  M9610213
 TI    Limited TCR V beta usage of infiltrating T cells in synovial tissues
       from patients with HTLV-I associated arthropathy.
 DT    9601
 AU    Ohshima K; Kondo S; Yoshida T; Kikuchi M; Shibata T; Sumiyoshi Y;
       Takeshita M; Department of Pathology, School of Medicine, Fukuoka
       University,; Japan.
 SO    Pathol Res Pract. 1995 Mar;191(2):148-55. Unique Identifier : AIDSLINE
       MED/96053618
 AB    Human T cell lymphotropic virus type-I (HTLV-I) is the etiologic agent
       of adult T cell leukemia/lymphoma and recently has also been suggested
       to be involved in chronic arthritis. The synovia of patients with
       rheumatoid arthritis (RA) contains activated T lymphocytes, with a
       restricted expression of T cell receptor (TCR) variable (V) beta gene
       segments. To characterize the T-cell populations of RA among HTLV-I
       carriers and noncarriers, we performed the immunohistochemical staining
       of CD4 and CDB, as well as a reverse transcription polymerase chain
       reaction (RT-PCR) to estimate the proportion of TCR beta RNA containing
       any particular V elements on the synovial specimens. In all but one
       HTLV-I carrier, the proviral DNA and/or RNA expression of HTLV-I was
       detected in the synovium. The CD4-positive cells proliferated markedly
       in the HTLV-I carriers compared with the noncarriers. In contrast to
       mononuclear cells in the peripheral blood, synovial T cells expressed
       only a few V beta transcripts, and no definite difference was observed
       between the carriers and the noncarriers. These results suggest that a
       common major antigen associated with the pathogenesis of RA may thus
       selectively interact with the V beta component of the TCR. Using RT-PCR,
       we studied the expression of the recombination-activating gene-1
       (RAG-1), which was used in the V(D)J recombination of immunoglobulin and
       TCR genes. In all cases, RAG-1 was transcripted. The results supported
       the possibility that the extrathymic development of the selected TCR V
       beta T cells occurred in the synovia.
 DE    Adult  Aged  Arthritis, Infectious/IMMUNOLOGY/METABOLISM/*PATHOLOGY
       Base Sequence  CD4-Positive T-Lymphocytes/*METABOLISM  CD8-Positive
       T-Lymphocytes/*METABOLISM  Female  Gene Products, rex  Human  HTLV-I
       Infections/*PATHOLOGY  Male  Middle Age  Molecular Sequence Data
       Proteins/ANALYSIS  Proviruses/GENETICS  Receptors, Antigen, T-Cell,
       alpha-beta/*ANALYSIS/IMMUNOLOGY  Support, Non-U.S. Gov't  Synovial
       Membrane/CHEMISTRY/IMMUNOLOGY/*PATHOLOGY  Trans-Activation (Genetics)
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

