       Document 0130
 DOCN  M9610130
 TI    Changes to alveolar macrophage phenotype in HIV infected individuals
       with normal CD4 counts and no respiratory disease.
 DT    9601
 AU    Lipman MC; Johnson MA; Bray DH; Poulter LH; Department of Clinical
       Immunology, Royal Free Hospital and School; of Medicine, London, UK.
 SO    Thorax. 1995 Jul;50(7):777-81. Unique Identifier : AIDSLINE MED/96014681
 AB    BACKGROUND--It has previously been shown that HIV infected individuals
       with pneumonitis have identifiable abnormalities in alveolar macrophages
       obtained by bronchoalveolar lavage (BAL). In particular, alterations in
       the expression of alveolar macrophage surface antigens associated with
       macrophage function have been reported. To determine whether these
       changes reflect HIV infection or the respiratory episode itself, a
       population of HIV infected patients with no respiratory disease was
       studied. METHODS--Twenty two HIV antibody positive individuals with a
       peripheral blood CD4 count of > 400/microliters and 10 healthy volunteer
       controls underwent bronchoscopy and BAL. Cytospin preparations from the
       recovered cells were stained using immunoperoxidase and double
       immunofluorescence techniques with monoclonal antibodies RFD1, RFD7,
       EBM11/CD68 (mature macrophages), UCHM1/CD14 (monocyte marker), and
       HLA-DR (RFDR1). Differential cell counts were also performed.
       RESULTS--There was an increase in overall alveolar macrophage HLA-DR
       expression in the HIV population. This was not reflected in a change in
       the percentage of cells staining CD14 (monocytes) or CD68 (mature
       macrophages) positive. The relative proportions of cells staining RFD1 +
       RFD7- (inducer cells), RFD1 - RFD7+ (effector cells), and RFD1 + RFD7+
       (suppressive cells) were unchanged between HIV and control groups.
       CONCLUSIONS--In a population of HIV infected individuals with normal CD4
       counts and no respiratory disease there was an increase in overall
       alveolar macrophage HLA-DR expression which occurred independently of
       any alteration in the relative proportions of alveolar macrophage
       subpopulations.
 DE    beta 2-Microglobulin/ANALYSIS  Adult  Antigen-Presenting Cells
       AIDS-Related Complex/COMPLICATIONS  Cell Count  CD4 Lymphocyte Count
       Female  Human  HIV Infections/*IMMUNOLOGY  HLA-DR Antigens/*IMMUNOLOGY
       Lymphocyte Count  Macrophages, Alveolar/*IMMUNOLOGY  Male  Support,
       Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

