       Document 0112
 DOCN  M9610112
 TI    Role of reactive nitrogen intermediates in the regulation of allogeneic
       skin graft survival in mice after portal vein pretransplant transfusion.
 DT    9601
 AU    Gorczynski RM; Rossi-Bergman B; Sullivan B; Chen Z; MRC Program Project
       Group, Department of Surgery, Toronto General; Hospital, Ontario,
       Canada.
 SO    Transplantation. 1995 Oct 15;60(7):707-13. Unique Identifier : AIDSLINE
       MED/96029807
 AB    C3H/HeJ mice received multiple minor histoincompatible skin grafts
       (B10.BR) after portal or lateral tail vein injection of irradiated
       B10.BR spleen cells. Some mice were additionally injected with a
       competitive inhibitor of nitric oxide synthesis
       (NG-monomethyl-L-arginine [NMMA]). Skin graft survival was extended
       following portal venous immunization, and further enhanced by NMMA. Both
       treatments produced decreased production of nitrate/nitrite in vivo, and
       were associated with enhanced expression of mRNA in vivo for type 2
       cytokines (interleukins 4 and 10), as well as increased synthesis of the
       latter on restimulation in vitro. Inducible nitric oxide synthase gene
       expression was up-regulated both in the local mucosal immune system
       (intraepithelial lymphocytes) and systemically (spleen) following
       antigen challenge by the portal vein or by gavage, with or without
       additional NMMA treatment. In contrast, when we studied a possible
       alternate in vivo source of nitric oxide production, we found that
       endothelial cell nitric oxide synthase expression was reproducibly
       up-regulated only in splenic tissue after combined oral (or portal)
       immunization and NMMA, and was not up-regulated in tissues local to the
       site of injection (intraepithelial lymphocytes).
 DE    Animal  Arginine/ANALOGS & DERIVATIVES/PHARMACOLOGY  Base Sequence  Cell
       Transplantation/*PHYSIOLOGY  Cytokines/BIOSYNTHESIS  Enzyme Activation
       Enzyme Induction  Graft Survival/DRUG EFFECTS/IMMUNOLOGY
       Isoantigens/PHARMACOLOGY  Mice  Mice, Inbred Strains  Molecular Sequence
       Data  Nitric Oxide/ANTAGONISTS & INHIB/BIOSYNTHESIS  Nitric-Oxide
       Synthase/GENETICS/METABOLISM  Nitrogen/*PHYSIOLOGY  Portal Vein  RNA,
       Messenger/METABOLISM  Skin Transplantation/*IMMUNOLOGY
       Spleen/*CYTOLOGY/IMMUNOLOGY  Th2 Cells/METABOLISM  Time Factors  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

