       Document 0103
 DOCN  M9610103
 TI    Serial passage of microglial SIV results in selection of homogeneous env
       quasispecies in the brain.
 DT    9601
 AU    Lane TE; Buchmeier MJ; Watry DD; Jakubowski DB; Fox HS; Department of
       Neuropharmacology, Scripps Research Institute, La; Jolla, California
       92037, USA.
 SO    Virology. 1995 Oct 1;212(2):458-65. Unique Identifier : AIDSLINE
       GENBANK/U19595
 AB    The pathogenic effects of HIV include infection of the central nervous
       system (CNS) which can result in cognitive and motor dysfunction. Simian
       immunodeficiency virus (SIV) infection of rhesus macaques provides an
       excellent model of HIV-induced disease. We have achieved a reproducible
       infection of the CNS using a stock of virus obtained by serial passage
       of microglia-associated SIV. Since the envelope genes of both HIV and
       SIV encode determinants important in viral pathogenesis, and the
       variability inherent in these viruses provides a molecular footprint of
       viral quasispecies, we analyzed the viral env sequences resulting from
       this serial passage. SIV env sequences were analyzed by direct PCR
       amplification of DNA isolated from microglia from infected animals.
       Nucleotide sequence comparison reveals that serial passage of
       microglia-associated SIV resulted in divergence from the donor stock of
       virus. Furthermore, an enrichment of unique env quasispecies which is
       maintained through the serial passage was found in the diseased brains.
 DE    Amino Acid Sequence  Animal  Base Sequence  Cloning, Molecular
       Consensus Sequence/GENETICS  DNA, Viral/GENETICS  Encephalitis,
       Viral/VIROLOGY  Genes, env/*GENETICS  Lymph Nodes/VIROLOGY  Macaca
       mulatta  Microglia/*VIROLOGY  Molecular Sequence Data  Sequence
       Analysis, DNA  Sequence Homology, Amino Acid  Serial Passage  Simian
       Acquired Immunodeficiency Syndrome/*VIROLOGY  Support, U.S. Gov't,
       P.H.S.  SIV/*GENETICS/PATHOGENICITY  Variation (Genetics)/GENETICS
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

