       Document 0102
 DOCN  M9610102
 TI    Analysis of herpes simplex virus-specific T cells in the murine female
       genital tract following genital infection with herpes simplex virus type
       2.
 DT    9601
 AU    Milligan GN; Bernstein DI; Division of Clinical Virology, James N.
       Gamble Institute of; Medical Research, Cincinnati, Ohio 45219, USA.
 SO    Virology. 1995 Oct 1;212(2):481-9. Unique Identifier : AIDSLINE
       MED/96010221
 AB    A murine model of genital infection with a thymidine kinase-deficient
       (tk-) strain of herpes simplex virus type 2 (HSV-2) was utilized to
       examine the development of the local T cell response in the genital
       mucosa and draining genital lymph nodes (gLN). HSV-specific
       cytokine-secreting T cells were detected in the gLN 4 days
       postintravaginal inoculation but not in the urogenital tract or spleen
       until 5 days postinoculation, suggesting the cellular immune response
       originates in the gLN. More CD4+ than CD8+ gLN T cells were detected by
       flow cytometric analysis following primary vaginal inoculation and the
       majority of HSV-specific gLN T cells detected by ELISPOT were CD4+ and
       Th1-like based on secretion of IFN gamma and not IL-4 or IL-5. A similar
       population of HSV-specific memory T cells persisted in the genital tract
       2 months following HSV-2 tk- genital inoculation. These data suggest
       that the urogenital cellular immune response elicited in mice following
       genital inoculation with HSV-2 tk- is predominantly CD4+ and Th1-like,
       resembling that observed in humans. The results of this study are
       important for the rational design of vaccines capable of inducing
       protective immunity in the genital tract.
 DE    Animal  Antigens, CD3/ANALYSIS  CD4-CD8 Ratio  CD4-Positive
       T-Lymphocytes/*IMMUNOLOGY  Female  Genitalia,
       Female/*IMMUNOLOGY/VIROLOGY  Herpes Genitalis/*IMMUNOLOGY/VIROLOGY
       Herpesvirus 2, Human/*IMMUNOLOGY  Human  Immunologic Memory/IMMUNOLOGY
       Interferon Type II/SECRETION  Lymph Nodes/IMMUNOLOGY  Lymphocyte
       Transformation  Mice  Mice, Inbred BALB C  Mucous
       Membrane/IMMUNOLOGY/VIROLOGY  Spleen/IMMUNOLOGY  Support, U.S. Gov't,
       P.H.S.  T-Lymphocyte Subsets/*IMMUNOLOGY  T-Lymphocytes,
       Cytotoxic/IMMUNOLOGY  Th1 Cells/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

