       Document 0101
 DOCN  M9610101
 TI    5' regions of HIV-1 RNAs are not sufficient for encapsidation:
       implications for the HIV-1 packaging signal.
 DT    9601
 AU    Berkowitz RD; Hammarskjold ML; Helga-Maria C; Rekosh D; Goff SP;
       Department of Biochemistry and Molecular Biophysics, Columbia;
       University, New York, New York 10032, USA.
 SO    Virology. 1995 Oct 1;212(2):718-23. Unique Identifier : AIDSLINE
       MED/96010245
 AB    The location and nature of the HIV-1 packaging signal are largely
       unknown, despite several genetic and biochemical mutational analyses. In
       this report we present our attempts to define a minimal HIV-1 packaging
       signal through the generation of test RNAs containing small blocks of
       HIV-1 sequences. We constructed RNAs differing in the position and
       identity of the HIV-1 sequence and the segments of heterologous
       sequences. However, none of the vectors were efficiently, encapsidated
       by wild-type HIV-1 virions. These results contrast those of Moloney
       murine leukemia virus and Rous sarcoma virus, where small viral segments
       mediate the efficient encapsidation of heterologous RNAs. The results
       suggest that the HIV-1 packaging signal may be extremely dispersed or
       heavily context-dependent.
 DE    Animal  Base Sequence  Cell Line  Cercopithecus aethiops
       Cytoplasm/VIROLOGY  Genetic Vectors/GENETICS  Human
       HIV-1/GENETICS/*PHYSIOLOGY  RNA Splicing  RNA, Viral/ANALYSIS/*GENETICS
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  Virion/CHEMISTRY
       Virus Assembly/*GENETICS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

