       Document 0100
 DOCN  M9610100
 TI    Identification of hypervariable and conserved regions in the surface
       envelope gene in the bovine lentivirus.
 DT    9601
 AU    Suarez DL; Whetstone CA; National Animal Disease Center, USDA,
       Agriculture Research; Service, Ames, Iowa 50010, USA.
 SO    Virology. 1995 Oct 1;212(2):728-33. Unique Identifier : AIDSLINE
       GENBANK/L43129
 AB    The surface envelope (SU) gene of nine different isolates of the bovine
       lentivirus (BIV) were compared for nucleotide and deduced amino acid
       (aa) sequence diversity. Analyses were done both on isolates derived
       from the original reference strain, R29, and on field isolates of BIV.
       Six conserved and six hypervariable regions were identified. Many of the
       hypervariable regions were located in areas predicted to be on the
       surface of the SU protein. The SU gene comparison among all isolates
       showed up to a 50% aa sequence divergence. When a conserved region of
       the reverse transcriptase gene was compared among eight of the isolates,
       there was less than 11% aa sequence divergence. When comparing all
       isolates, the greatest size differences in the SU gene are observed in
       the 2nd hypervariable region (V2) with up to a 104-aa difference between
       the largest and smallest variant. R29-106, an infectious molecular clone
       of the original isolate of BIV, has an 87-bp deletion in V2 as compared
       to prototype isolate R29-127. All R29-derived isolates sequenced for
       this study had a SU gene size similar to R29-106. The four field
       isolates sequenced for this study had SU genes larger than R29-127.
       R29-derived isolates may not be representative of BIV currently present
       in United States cattle.
 DE    Amino Acid Sequence  Animal  Base Sequence  Cattle  *Conserved Sequence
       Genes, env/*GENETICS  Lentiviruses, Bovine/*GENETICS  Molecular Sequence
       Data  Sequence Analysis, DNA  Sequence Homology, Amino Acid  Sequence
       Homology, Nucleic Acid  Support, U.S. Gov't, Non-P.H.S.  Support, U.S.
       Gov't, P.H.S.  Variation (Genetics)/*GENETICS  Viral Envelope
       Proteins/CHEMISTRY/*GENETICS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

