       Document 0081
 DOCN  M9610081
 TI    Clonal expansion of T cells and HIV genotypes in microdissected splenic
       white pulps indicates viral replication in situ and infiltration of
       HIV-specific cytotoxic T lymphocytes.
 DT    9601
 AU    Cheynier R; Henrichwark S; Hadida F; Pelletier E; Oksenhendler E; Autran
       B; Wain-Hobson S; Unite de Retrovirologie Moleculaire, Institut Pasteur,
       Paris,; France.
 SO    Adv Exp Med Biol. 1995;374:173-82. Unique Identifier : AIDSLINE
       MED/96047247
 AB    Human immunodeficiency virus (HIV) replication and T cell proliferation
       was investigated in situ by a PCR based analysis of individual
       microdissected splenic white pulps. Founder effects, revealed by an
       exquisite compartmentalization of HIV genotypes and T cells, indicated
       the recruitment of latently infected CD4+ T cells through highly
       localized antigen presentation, rather than the infection of CD4+ T
       lymphoblasts by blood borne virus or immune complexes. HIV infected
       white pulps could be infiltrated by HIV specific cytotoxic T
       lymphocytes, so implicating them in CD4+ T cell destruction in vivo.
       Together these data describe an iterative and deleterious mechanism of
       antigen driven T cell recruitment and activation, HIV replication and
       spread, with consequent destruction of the newly infected cells.
 DE    Amino Acid Sequence  Antibody Specificity  Clone Cells  Dissection
       Genotype  Human  HIV/GENETICS/*PHYSIOLOGY  *HIV Antibodies
       Micromanipulation  Molecular Sequence Data  Spleen/*PATHOLOGY  Support,
       Non-U.S. Gov't  *T-Lymphocytes, Cytotoxic  *Virus Replication  JOURNAL
       ARTICLE  REVIEW  REVIEW, TUTORIAL

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

