       Document 0068
 DOCN  M9610068
 TI    A highly sensitive and rapid procedure for direct PCR detection of
       Leishmania infantum within human peripheral blood mononuclear cells.
 DT    9601
 AU    Ravel S; Cuny G; Reynes J; Veas F; Laboratoire Retrovirus-Parasites,
       Institut Francais de; Recherche Scientifique pour le Developpement en
       Cooperation; (ORSTOM), Montpellier, France.
 SO    Acta Trop. 1995 Jun;59(3):187-96. Unique Identifier : AIDSLINE
       MED/96047638
 AB    We have developed a highly sensitive, simple and rapid procedure to
       detect Leishmania infantum within human macrophages. It only requires
       ficoll preparation of peripheral blood mononuclear cells from the
       patient, and their direct use for Leishmania kDNA amplification by
       polymerase chain reaction. Under these conditions, about one parasite
       can be detected in a one million human cell environment. Results,
       including those of a hybridization step to confirm the diagnosis
       specificity, are obtained with 24 h, a very short period as compared to
       current diagnostic methods. This procedure is of particular interest for
       early detection and early drug treatment of leishmaniasis, especially in
       the case of HIV coinfection. Furthermore, the method could be useful for
       monitoring the efficiency of new leishmaniasis treatments in infected
       patients.
 DE    Animal  Base Sequence  Human  Leishmania infantum/*ISOLATION & PURIF
       Leukocytes, Mononuclear/*PARASITOLOGY  Macrophages/PARASITOLOGY
       Molecular Sequence Data  *Polymerase Chain Reaction  Sensitivity and
       Specificity  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

