       Document 0041
 DOCN  M9610041
 TI    Bacterial expression of the caprine arthritis-encephalitis virus gag and
       env proteins and their use in enzyme-linked immunosorbent assay.
 DT    9601
 AU    Clavijo A; Thorsen J; Department of Veterinary Microbiology and
       Immunology, University; of Guelph, Ontario, Canada.
 SO    Am J Vet Res. 1995 Jul;56(7):841-8. Unique Identifier : AIDSLINE
       MED/96039854
 AB    The core protein and the transmembrane protein, encoded for the
       structural genes gag and env, respectively, of caprine
       arthritis-encephalitis virus were amplified by use of polymerase chain
       reaction, cloned into a pGEX-2T vector, and expressed in Escherichia
       coli as fusion proteins with the glutathione S-transferase at their
       C-terminus. The recombinant proteins were purified and evaluated by use
       of an ELISA. Sera from 269 goats were tested, and the results were
       compared with those obtained by use of immunoblot analysis. When results
       from both recombinant ELISA (r-ELISA) were compared, it appeared that
       the transmembrane glycoprotein was more immunoreactive than the core
       protein, because it was recognized by a higher percentage of sera from
       infected goats. When results of the 2 ELISA (p28 r-ELISA and p40
       r-ELISA) were combined in parallel, they were comparable to those of the
       immunoblot test, with sensitivity of 100% and specificity of 98.3%. It
       was also found that use of both r-ELISA makes it possible to compare the
       variable immunoreactivity against gag and env viral antigens, which may
       be correlated with the disease state. The r-ELISA, using core and
       transmembrane proteins, appears to be highly sensitive and specific for
       detection of antibodies against caprine arthritis-encephalitis virus.
 DE    Animal  Arthritis-Encephalitis Virus, Caprine/GENETICS/*ISOLATION &
       PURIF  Base Sequence  Cloning, Molecular  Comparative Study  DNA Primers
       Electrophoresis, Polyacrylamide Gel  Enzyme-Linked Immunosorbent
       Assay/METHODS  Escherichia coli  False Positive Reactions  Gene
       Products, env/ANALYSIS/*BIOSYNTHESIS/ISOLATION & PURIF  Gene Products,
       gag/ANALYSIS/*BIOSYNTHESIS/ISOLATION & PURIF  *Goat Diseases  Goats
       Lentivirus Infections/DIAGNOSIS/*VETERINARY  Molecular Sequence Data
       Polymerase Chain Reaction  Recombinant Fusion
       Proteins/ANALYSIS/BIOSYNTHESIS/ISOLATION &  PURIF  Reproducibility of
       Results  Sensitivity and Specificity  Support, Non-U.S. Gov't  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

