       Document 0039
 DOCN  M9610039
 TI    HIV-1 messenger RNA in peripheral blood mononuclear cells as an early
       marker of risk for progression to AIDS [comment]
 DT    9601
 AU    Saksela K; Stevens CE; Rubinstein P; Taylor PE; Baltimore D; Rockefeller
       University, New York, New York, USA.
 SO    Ann Intern Med. 1995 Nov 1;123(9):641-8. Unique Identifier : AIDSLINE
       MED/96011271
 CM    Comment on: Ann Intern Med 1995 Nov 1;123(9):726-7
 AB    OBJECTIVE: To establish human immunodeficiency virus type 1 (HIV-1)
       messenger RNA (mRNA) expression in peripheral blood mononuclear cells as
       a marker of risk for progression to the acquired immunodeficiency
       syndrome (AIDS) in a large cohort of HIV-infected persons followed for a
       prolonged period. DESIGN: Retrospective testing of cryopreserved, coded
       specimens. SETTING: Research laboratories at the New York Blood Center
       and the Rockefeller University. PATIENTS: 150 homosexual men infected
       with HIV-1 who did not have an AIDS diagnosis at the time of testing.
       MEASUREMENTS: Multiply spliced and unspliced HIV-1 mRNAs in total
       peripheral blood mononuclear cell RNA were quantitated using reverse
       transcriptase-initiated polymerase chain reaction (PCR) and compared
       with other laboratory data and clinical outcome during the subsequent 8
       years. RESULTS: Although HIV-1 mRNA expression generally correlated with
       immunologic status, it was associated with future disease progression
       independently of CD4+ cell counts or their rate of decrease at the time
       of sampling. The association of HIV-1 mRNA with disease progression in
       persons with CD4+ cell counts higher than the median (> 624 cells/mm3)
       was particularly noteworthy; further variation in the CD4+ cell counts
       within this group was not prognostically significant. CONCLUSIONS: The
       expression of HIV-1 mRNA in peripheral blood mononuclear cells is a
       strong independent marker for future HIV disease progression, even in
       persons with normal T-cell subsets.
 DE    Acquired Immunodeficiency Syndrome/BLOOD/*VIROLOGY  Adult  Biological
       Markers/BLOOD  CD4 Lymphocyte Count  Disease Progression  Human  HIV
       Seropositivity/BLOOD/*VIROLOGY  HIV-1/*GENETICS  Male  Retrospective
       Studies  RNA, Messenger/*BLOOD  Support, Non-U.S. Gov't  Support, U.S.
       Gov't, P.H.S.  COMMENT  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

