       Document 0862
 DOCN  M9440862
 TI    Host-related DNA sequences are localized in the body of schistosome
       adults.
 DT    9404
 AU    Irie Y; Iwamura Y; Institute of Basic Medical Sciences, University of
       Tsukuba,; Ibaraki-ken, Japan.
 SO    Parasitology. 1993 Dec;107 ( Pt 5):519-28. Unique Identifier : AIDSLINE
       MED/94126274
 AB    The localization of host (mouse)-related DNA sequences in the adults and
       cercariae of Schistosoma japonicum and S. mansoni was examined by in
       situ hybridization using 32P-labelled probes. The hybridization signals
       to the sequences of the mouse type C and type A retroviruses were
       clearly observed in the subtegumental layer and inner tissues of S.
       japonicum adults. In contrast, it was hard to find any signals to these
       sequences in S. mansoni adults. Distinct signals to the env-specific
       region of the mouse ecotropic type C retrovirus were observed in the
       subtegumental layer and inner tissues of S. mansoni adults. No signal to
       the sequence of the mouse type B retrovirus was found on the sections of
       either schistosome adult. The signals to the sequence of the genomic
       clone SmM51 derived from male S. mansoni were found in the tissues of
       both the schistosome adults. The signals to the sequences of the mouse
       type A and env-specific region of ecotropic type C retroviruses were not
       seen in the cercariae of each species, whereas the signals to the
       sequence of SmM51 were detected in S. mansoni cercariae. These
       observations suggest that host-related DNA sequences might be
       incorporated unequally into the schistosome genome during development in
       the final host.
 DE    Animal  DNA/*ANALYSIS  DNA Probes  DNA, Viral/ANALYSIS  Female  Genes,
       env  In Situ Hybridization  Male  Mice  Mice, Inbred AKR  Mice, Inbred
       ICR  Organ Specificity  Retroviridae/*ISOLATION & PURIF  Schistosoma
       japonicum/CYTOLOGY/*GENETICS  Schistosoma mansoni/CYTOLOGY/*GENETICS
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

