       Document 0738
 DOCN  M9440738
 TI    A non-lymphoma idiotype is indicative and predictive for B cell
       malignancies in AIDS.
 DT    9404
 AU    Herndier B; McGrath M; Abbey N; Wang HT; Ng V; Dorigo O; Kaplan L;
       Muller S; Kohler H; Department of Pathology, San Francisco General
       Hospital,; University of California.
 SO    Hybridoma. 1993 Oct;12(5):529-37. Unique Identifier : AIDSLINE
       MED/94131484
 AB    The 1F7 idiotype previously defined (J Immunol 147:933;1991 and Eur J
       Immunol 22:1749;1992) is expressed on antibodies reactive to different
       proteins of HIV (gp41, p24, gp120). Since serum levels of 1F7 (IgM or
       IgG) are significantly higher in patients with HIV lymphoma as opposed
       to HIV-infected individuals, normal controls and non-HIV lymphoma
       patients, we hypothesized the B-cell neoplasms were the source of the
       idiotype. However, immunohistochemistry on cryostat sections revealed no
       1F7 idiotype signal on neoplastic B-cells nor tumor infiltrative T-cells
       (n = 8). Interestingly, reactive lymphocytosis adjacent to tumor masses
       and reactive follicular hyperplastic controls (n = 5) exhibited
       significant 1F7 reactivity. The reactivity appeared in paracortical and
       perifollicular lymphoid regions, predominantly regions of B, T and
       antigen presenting cells in lymph nodes or tonsils. A survey of
       electrophoretically defined paraproteins with anti-HIV specificities
       derived from HIV-infected patients showed no Western blot reactivity
       with the 1F7 anti-idiotypic antibody. Therefore, the idiotype does not
       appear to be a direct product of B-cell neoplasia or abnormal B-cell
       proliferation, but is produced by B cell clones responding to HIV
       infection. This high level of serum 1F7 reactivity could be an important
       clue in the pathogenesis of HIV lymphomas and confers a highly
       predictive serological test for HIV lymphoma.
 DE    Animal  Antibodies, Anti-Idiotypic/BLOOD  B-Lymphocytes/IMMUNOLOGY
       Human  IgG/BLOOD  IgM/BLOOD  *Immunoglobulin Idiotypes/METABOLISM
       Immunohistochemistry  Lymphoid Tissue/IMMUNOLOGY  Lymphoma,
       AIDS-Related/*IMMUNOLOGY  Mice  Paraproteins/IMMUNOLOGY  Support, U.S.
       Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

