       Document 0711
 DOCN  M9440711
 TI    Involvement of CD4 in interleukin-6 secretion by U937 monocytic cells
       stimulated with the lectin jacalin.
 DT    9404
 AU    Taimi M; Dornand J; Nicolas M; Marti J; Favero J; INSERM U65, Universite
       Montpellier II, Sciences et Techniques du; Languedoc, France.
 SO    J Leukoc Biol. 1994 Feb;55(2):214-20. Unique Identifier : AIDSLINE
       MED/94132728
 AB    The lectin jacalin is mitogenic for CD4 expressing T lymphocytes,
       interacts with the CD4 molecule, and inhibits HIV infection of CD4+
       cells. In the present study the effect of jacalin was tested on cells
       from the monocyte/macrophage lineage that also express the CD4 molecule.
       We used CD4+ promyelomonocytic U937 cells differentiated towards the
       monocytic/macrophage lineage with either a mixture of two physiological
       agents, retinoic acid (RA) and 1 alpha,25-dihydroxyvitamin D3 (VD), or
       the exogenous drug phorbol myristate acetate (PMA). The cells resulting
       from these treatments differed in term of CD4 expression. We focused our
       attention on interleukin-6 (IL-6) production, which implies an
       activation of the cells differentiated along both pathways. In CD4+
       RA/VD-treated cells, jacalin induced a 10-fold higher IL-6 secretion
       than did lipopolysaccharide (LPS). This jacalin-induced IL-6 production
       was inhibited by agents interacting with CD4 (anti-CD4 mAbs and HIV
       recombinant gp120) or by recombinant soluble CD4. In contrast, the CD4-
       PMA-differentiated U937 cells did not secrete any IL-6 upon jacalin
       treatment, while they demonstrated a response to LPS similar to that of
       the RA/VD-differentiated cells. Together with the fact that jacalin
       interacts with CD4, these results provide evidence of the involvement of
       a CD4 dependent pathway in IL-6 production.
 DE    Antibodies, Monoclonal/PHARMACOLOGY  Antigens, CD/*PHYSIOLOGY  Antigens,
       CD4/*PHYSIOLOGY  Biological Assay  Calcitriol/PHARMACOLOGY  Cell
       Differentiation  Cell Line  Chemiluminescence  Flow Cytometry  Human
       HIV Envelope Protein gp120/PHARMACOLOGY  Interferon
       Inducers/*PHARMACOLOGY  Interleukin-1/BIOSYNTHESIS
       Interleukin-6/*BIOSYNTHESIS/SECRETION  Lectins/*PHARMACOLOGY  Leukemia,
       Promyelocytic, Acute  Lipopolysaccharides/PHARMACOLOGY  Monocytes/DRUG
       EFFECTS/IMMUNOLOGY/*METABOLISM  Recombinant Proteins/PHARMACOLOGY
       Support, Non-U.S. Gov't  Tetradecanoylphorbol Acetate/PHARMACOLOGY
       Tretinoin/PHARMACOLOGY  Tumor Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

