       Document 0490
 DOCN  M9440490
 TI    Urinary excretion of modified nucleosides as biological marker of RNA
       turnover in patients with cancer and AIDS.
 DT    9404
 AU    Nakano K; Nakao T; Schram KH; Hammargren WM; McClure TD; Katz M;
       Petersen E; Life Science Division, Church of Perfect Liberty, Osaka,
       Japan.
 SO    Clin Chim Acta. 1993 Sep 30;218(2):169-83. Unique Identifier : AIDSLINE
       MED/94139052
 AB    Using boronate gel affinity chromatography and reversed-phase
       high-performance liquid chromatography (RP-HPLC), a method for the
       simultaneous determination of 12 urinary modified nucleosides has been
       developed. The RP-HPLC fractions were identified by gas
       chromatography/mass spectrometry analysis. The HPLC quantitation of
       urinary nucleoside levels before and after surgery of cancer patients
       suggested that urinary 5'-deoxy-5'-methylthioadenosine and
       N-[(9-beta-D-ribofuranosyl-9H-purine-6-yl) carbamoyl]-L-threonine (t6A)
       levels were helpful in monitoring therapeutic effects in cancer
       patients. From the fact that molar ratios of urinary
       N2,N2-dimethylguanosine (m2 2G)pseudouridine (psi) and t6A/psi in cancer
       patients were lower than those of normal or post-surgical cancer
       patients, the increase of rRNA content in cancer tissues growing rapidly
       was estimated using the stoichiometric relationship between the ratio of
       the number of residues of their modified nucleoside in RNAs and the
       proportion of rRNA to total RNAs in average tissues of whole body.
       Furthermore, from the estimation of RNA turnover using urinary
       nucleoside levels, it was found that the half-lives of rRNA rather than
       tRNA of patients with cancer and those of both RNAs in the case of
       acquired immunodeficiency syndrome (AIDS) were extremely short compared
       with those of the normal. Thus, we discovered that the selected urinary
       modified nucleosides were very useful as a biological marker of
       whole-body RNA turnover in patients with cancer and AIDS.
 DE    Acquired Immunodeficiency Syndrome/*URINE  Aged  Aged, 80 and over
       Chromatography, Affinity  Chromatography, High Pressure Liquid  Colonic
       Neoplasms/URINE  Female  Half-Life  Human  Male  Mass Fragmentography
       Middle Age  Neoplasms/*URINE  Nucleosides/*URINE  Rectal Neoplasms/URINE
       RNA/*METABOLISM  RNA, Ribosomal/METABOLISM  RNA, Transfer/METABOLISM
       Stomach Neoplasms/URINE  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

