       Document 0238
 DOCN  M9440238
 TI    Sequence variation within the human immunodeficiency virus V3 loop at
       seroconversion.
 DT    9404
 AU    Ait-Khaled M; Emery VC; Division of Communicable Diseases, Royal Free
       Hospital School of; Medicine, London, United Kingdom.
 SO    J Med Virol. 1993 Dec;41(4):270-4. Unique Identifier : AIDSLINE
       MED/94149438
 AB    Analysis of the HIV-1 V3 quasispecies present in an individual at the
       time of seroconversion was carried out. The polymerase chain reaction
       (PCR) was used to amplify proviral HIV-1 DNA extracted from peripheral
       blood mononuclear cells from a patient who was viraemic (p24 = 15 pg/ml)
       and had an equivocal HIV-1 antibody status. The PCR products were cloned
       and the DNA sequence determined for 15 clones. These data showed that
       the V3 region contained only limited sequence heterogeneity with a major
       variant accounting for 66% of the protein quasispecies present. The
       protein sequence of the principal neutralising domain on all species
       contained the relatively rare GPGKTL motif rather than GPGRAF. The
       relevance of these data for early stages of HIV infection are discussed.
 DE    Amino Acid Sequence  Base Sequence  Cloning, Molecular  DNA, Viral/BLOOD
       Genes, env/*GENETICS  Human  HIV Envelope Protein gp120/*GENETICS  *HIV
       Seropositivity  HIV-1/*GENETICS  Molecular Sequence Data  Mutation
       Peptide Fragments/*GENETICS  Polymerase Chain Reaction  Sequence
       Alignment  Sequence Analysis  Support, Non-U.S. Gov't  Viremia  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

