       Document 0211
 DOCN  M9440211
 TI    Major histocompatibility complex-restricted CD8+ cytotoxic T lymphocytes
       from horses with equine infectious anemia virus recognize Env and Gag/PR
       proteins.
 DT    9404
 AU    McGuire TC; Tumas DB; Byrne KM; Hines MT; Leib SR; Brassfield AL;
       O'Rourke KI; Perryman LE; Department of Veterinary Microbiology and
       Pathology, Washington; State University, Pullman 99164-7040.
 SO    J Virol. 1994 Mar;68(3):1459-67. Unique Identifier : AIDSLINE
       GENBANK/X16988
 AB    Cytotoxic T lymphocytes (CTL) can control some viral infections and may
       be important in the control of lentiviruses, including human
       immunodeficiency virus type 1. Since there is limited evidence for an in
       vivo role of CTL in control of lentiviruses, dissection of immune
       mechanisms in animal lentiviral infections may provide needed
       information. Horses infected with equine infectious anemia virus (EIAV)
       a lentivirus, have acute plasma viremia which is terminated in
       immunocompetent horses. Viremic episodes may recur, but most horses
       ultimately control infection and become asymptomatic carriers. To begin
       dissection of the immune mechanisms involved in EIAV control, peripheral
       blood mononuclear cells (PBMC) from infected horses were evaluated for
       CTL to EIAV-infected cells. By using noninfected and EIAV-infected
       autologous equine kidney (EK) cells in 51Cr-release assays,
       EIAV-specific cytotoxic activity was detected in unstimulated PBMC from
       three infected horses. The EIAV-specific cytotoxic activity was major
       histocompatibility complex (MHC) restricted, as determined by assaying
       EIAV-infected heterologous EK targets, and was mediated by CD8+ T
       lymphocytes, as determined by depleting these cells by a panning
       procedure with an anti-CD8 monoclonal antibody. MHC-restricted CD8+ CTL
       in unstimulated PBMC from infected horses caused significant specific
       lysis of autologous EK cells infected with recombinant vaccinia viruses
       expressing EIAV genes, either env or gag plus 5' pol. The EIAV-specific
       MHC-restricted CD8+ CTL were detected in two EIAV-infected horses within
       a few days after plasma viremia occurred and were present after viremia
       was terminated. The detection of these immune effector cells in
       EIAV-infected horses permits further studies to determine their in vivo
       role.
 DE    Animal  Antigens, CD8/*IMMUNOLOGY  Equine Infectious Anemia/*IMMUNOLOGY
       Gene Products, env/GENETICS/*IMMUNOLOGY  Gene Products, gag/*IMMUNOLOGY
       Histocompatibility Antigens/IMMUNOLOGY  Horses  Male  Molecular Sequence
       Data  Recombinant Proteins/IMMUNOLOGY  Support, Non-U.S. Gov't  Support,
       U.S. Gov't, P.H.S.  T-Lymphocyte Subsets/IMMUNOLOGY  T-Lymphocytes,
       Cytotoxic/*IMMUNOLOGY  Vaccinia Virus/GENETICS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

