       Document 0207
 DOCN  M9440207
 TI    A new subtype of human immunodeficiency virus type 1 (MVP-5180) from
       Cameroon.
 DT    9404
 AU    Gurtler LG; Hauser PH; Eberle J; von Brunn A; Knapp S; Zekeng L; Tsague
       JM; Kaptue L; Max von Pettenkofer Institute, University of Munich,
       Germany.
 SO    J Virol. 1994 Mar;68(3):1581-5. Unique Identifier : AIDSLINE
       MED/94149848
 AB    A new subtype (MVP-5180) of human immunodeficiency virus type 1 (HIV-1)
       was isolated from a Cameroonian AIDS patient. MVP-5180 was grown in
       several human T-cell lines and the monocytic U937 line. MVP-5180 DNA
       could not be amplified by nested primer PCR with conventional env
       primers and could be only very faintly amplified with gag and pol
       primers. Most German, Ivoirian, and Malawian anti-HIV-1 sera reacted
       faintly or moderately with Env proteins in an MVP-5180 immunoblot,
       whereas some Cameroonian sera reacted strongly. Of HIV-1-infected
       Cameroonians, 8% were identified by serological methods as infected with
       MVP-5180; 7% were positive when MVP-5180-specific PCR env primers were
       used. DNA sequence analysis of MVP-5180 showed that its genetic
       organization was that of HIV-1, with 65% similarity to HIV-1 and 56%
       similarity to HIV-2 consensus sequences. The env gene of MVP-5180 had
       similarities to HIV-1 and HIV-2 of 53 and of 49%, respectively. V3 loop
       analysis identified a crown of Gly-Pro-Met-Arg by using cloned DNA and
       Gly-Pro-Leu-Arg by using PCR-amplified DNA, neither of which
       configuration has been described for other HIV strains. In an analysis
       of relationships, MVP-5180 occupied a position distant to all other
       HIV-1 strains, including the chimpanzee simian immunodeficiency virus
       type 1 SIVcpz and the Uganda virus U455, and closer to the HIV-1/HIV-2
       divergence node. MVP-5180, together with another Cameroonian isolate,
       ANT-70, constitutes a group subtype O of the most divergent HIV-1
       isolates yet identified. Characterization of MVP-5180 is important for
       understanding the natural history of the primate immunodeficiency
       viruses and for the development of vaccines and diagnostics.
 DE    Acquired Immunodeficiency Syndrome/EPIDEMIOLOGY/MICROBIOLOGY  Amino Acid
       Sequence  Base Sequence  Cameroon/EPIDEMIOLOGY  Cell Line  Comparative
       Study  Human  HIV Envelope Protein gp120/GENETICS
       HIV-1/*CLASSIFICATION/GENETICS/ISOLATION & PURIF  HIV-2/GENETICS
       Immunoblotting  Molecular Sequence Data  Polymerase Chain Reaction
       Prevalence  Sequence Analysis, DNA  Sequence Homology, Amino Acid
       T-Lymphocytes/CYTOLOGY/MICROBIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

