       Document 0188
 DOCN  M9440188
 TI    Induction of beta interferon by human immunodeficiency virus type 1 and
       its gp120 protein in human monocytes-macrophages: role of beta
       interferon in restriction of virus replication.
 DT    9404
 AU    Gessani S; Puddu P; Varano B; Borghi P; Conti L; Fantuzzi L; Belardelli
       F; Department of Virology, Istituto Superiore di Sanita, Rome,; Italy.
 SO    J Virol. 1994 Mar;68(3):1983-6. Unique Identifier : AIDSLINE
       MED/94149896
 AB    In vitro cultivated human monocytes show a time-dependent
       differentiation into macrophages, characterized by an increased
       expression of macrophage-specific antigens. Monocytes-macrophages were
       infected with human immunodeficiency virus type 1 strain Ba-L
       (HIV-1Ba-L) at different stages of differentiation. When 7-day cultured
       macrophages were infected in the presence of antibodies to beta
       interferon (IFN-beta), a significant increase in HIV-1 p24 release was
       detected. This effect was not observed in 1-day monocytes. This finding
       suggests that IFN-beta secreted by the infected macrophages inhibits p24
       release. Treatment of cultured macrophages with recombinant gp120
       (rgp120) protein resulted in the induction of IFN-beta mRNA and in an
       antiviral state to vesicular stomatitis virus. This rgp120-induced
       antiviral state was largely neutralized by antibodies to IFN-beta,
       whereas anti-IFN-alpha antibodies were ineffective. In cultured
       macrophages, 0.1 IU of IFN-beta per ml was sufficient to induce a marked
       inhibition of vesicular stomatitis virus yield, whereas this dose was
       ineffective in 1-day monocytes. These results indicate that (i) HIV-1
       (possibly in part through its gp120 protein) induces low levels of
       IFN-beta in macrophages and (ii) this IFN-beta is very effective in
       inducing an antiviral state in differentiated macrophages.
 DE    Cell Differentiation  Cells, Cultured  Comparative Study  Dose-Response
       Relationship, Drug  Gene Expression Regulation/DRUG EFFECTS  Human  HIV
       Core Protein p24/BIOSYNTHESIS  HIV Envelope Protein gp120/*PHARMACOLOGY
       HIV-1/GROWTH & DEVELOPMENT/*IMMUNOLOGY  Interferon Inducers/PHARMACOLOGY
       Interferon-beta/*BIOSYNTHESIS/IMMUNOLOGY/PHARMACOLOGY  Macrophages/DRUG
       EFFECTS/MICROBIOLOGY/*PHYSIOLOGY  Monocytes/DRUG
       EFFECTS/MICROBIOLOGY/*PHYSIOLOGY  RNA, Messenger/BIOSYNTHESIS  Support,
       Non-U.S. Gov't  Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

