       Document 1019
 DOCN  M9541019
 TI    Infectious virus with reduced cytopathogenicity resulting from
       persistent infection of normal lung fibroblasts by HIV type 1 strains.
 DT    9504
 AU    Dolei A; Serra C; Arca MV; Tilocca F; Riva E; Antonelli G; Dianzani F;
       Toniolo A; Institute of Microbiology and Virology, University of
       Sassari,; Italy.
 SO    AIDS Res Hum Retroviruses. 1994 Sep;10(9):1089-95. Unique Identifier :
       AIDSLINE MED/95127291
 AB    We asked whether HIV-1 had the capacity to establish a persistent
       infection of cultured human diploid fibroblasts. Human strains of normal
       diploid embryo lung fibroblasts were infected with HIV-1 of the
       HTLV-IIIB and HIV-1P1 strains. Infection was followed over time, to
       analyze HIV expression. Virus production (intra- and extracellular
       virus) was evaluated as follows: ability to form syncytia in the C8166 T
       cell line, production of p24 and other viral antigens (ELISA and
       indirect immunofluorescence), search for a gag sequence in cell DNA by
       the polymerase chain reaction followed by hybridization to an
       HIV-1-specific probe (SK19). Cell-free culture supernatant was used as a
       virus source to infect de novo fibroblasts and C8166 T cells. Infection
       of cultured fibroblasts with either the HTLV-IIIB or HIV-1P1 strain led
       regularly to the establishment of persistently infected cultures.
       Fibroblast cells were capable of continuous virus production for at
       least 10 months. The released virus was capable of reinfecting cultured
       fibroblasts and of producing cytopathic effects in the C8166 T cell
       line. However, when compared to wild-type strains, the infectious virus
       derived from fibroblasts showed a prolonged replication cycle and a
       decreased ability to form syncytia in the T cell line. Therefore, HIV-1
       can establish a persistent and productive infection in normal lung
       fibroblasts. The data are consistent with the hypothesis that in vivo,
       at least in the lung, fibroblasts may represent a virus reservoir and
       that infection of these cells may lead to the production of attenuated
       variants of HIV.
 DE    Cell Line  Embryo  Enzyme-Linked Immunosorbent Assay
       Fibroblasts/VIROLOGY  Giant Cells  Human  HIV
       Antigens/ANALYSIS/BIOSYNTHESIS  HIV Core Protein
       p24/ANALYSIS/BIOSYNTHESIS  HIV-1/*PHYSIOLOGY/*PATHOGENICITY  Lung
       Membrane Fusion  Polymerase Chain Reaction  Support, Non-U.S. Gov't
       T-Lymphocytes  *Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

