       Document 0950
 DOCN  M9540950
 TI    Depressed T cell reactivity to recall antigens in Crohn's disease before
       and after surgical resection.
 DT    9504
 AU    D'Haens G; Hiele M; Rutgeerts P; Geboes K; Ceuppens JL; Department of
       Internal Medicine, Leuven University Hospital,; Belgium.
 SO    Gut. 1994 Dec;35(12):1728-33. Unique Identifier : AIDSLINE MED/95129999
 AB    Earlier studies regarding possible primary immune disturbances
       participating in the pathogenesis of Crohn's disease yielded conflicting
       results. Peripheral blood lymphocyte subsets and lymphocyte
       proliferative responses to five soluble recall antigens and to the
       polyclonal stimulator phythaemagglutinin were therefore measured in 17
       patients with active Crohn's disease, before and six months after
       surgical resection of the inflamed intestine and in 16 healthy controls.
       Lymphocyte proliferation in response to all five recall antigens was
       significantly lower in patients than in controls. No significant
       differences with controls were detected after surgery. Addition of
       indomethacin to phythaemagglutinin stimulated lymphocyte cultures had a
       stronger proliferation enhancing effect in patients than in controls,
       resulting in comparable proliferative responses in both groups. When
       both indomethacin and prostaglandin E2 were added, inhibition of
       reactivity by prostaglandin E2 was stronger in patients' cultures. This
       suggests a higher sensitivity to inflammatory prostaglandins in Crohn's
       disease. The degree of lymphocyte stimulation by antigens correlated
       positively with the percentage of circulating memory T cells (CD 45
       RA-). The percentage of activated (HLA-DR+) CD8 cells was higher in
       patients than in controls. The CD4/CD8 ratio, which was not
       significantly different between patients and controls, correlated
       significantly with disease activity and characteristics, even in the
       postoperative phase. These findings suggest that immune abnormalities in
       Crohn's disease fluctuate with and are probably secondary to
       inflammatory activity.
 DE    Adult  Aged  Cell Division/DRUG EFFECTS  Crohn
       Disease/*IMMUNOLOGY/*SURGERY  CD4-CD8 Ratio  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  Dinoprostone/PHARMACOLOGY  Female  Human
       Immunocompetence  Immunologic Memory/*IMMUNOLOGY
       Indomethacin/PHARMACOLOGY  Lymphocyte Transformation  Male  Middle Age
       T-Lymphocyte Subsets/*IMMUNOLOGY  Time Factors  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

