       Document 0843
 DOCN  M9540843
 TI    Antibody to HIV-1 Tat protein inhibits the replication of virus in
       culture.
 DT    9504
 AU    Steinaa L; Sorensen AM; Nielsen JO; Hansen JE; Department of Infectious
       Disease, Hvidovre Hospital, University; of Copenhagen, Denmark.
 SO    Arch Virol. 1994;139(3-4):263-71. Unique Identifier : AIDSLINE
       MED/95134084
 AB    The HIV-1 transactivator protein Tat is essential for viral replication.
       Tat is released from infected cells and can be taken up and
       transactivate HIV-LTR in LTR-CAT transfected cell lines. The present
       study shows that the addition of monoclonal antibody to Tat in IIIB and
       MN-infected cultures reduces the HIV antigen production in a
       concentration dependent manner. These data suggest that external Tat
       might be important in the replication of HIV, exerting the effect in a
       paracrine fashion. Using 1 microgram/ml of anti-Tat antibody resulted in
       a decline of HIV antigen production to 33% and 45% of controls in IIIB
       and MN infected H9 cells, respectively. A time course experiment showed
       progressively increased inhibition of replication during 7 days of
       exposure to anti-Tat antibody, which could be due to increasing Tat
       concentration. The inhibitory effect of anti-Tat antibodies on the
       replication of HIV could play an important regulatory role during
       infection in vivo.
 DE    Antibodies, Monoclonal/IMMUNOLOGY  Cell Line  Gene Products,
       tat/IMMUNOLOGY/*PHYSIOLOGY  Human  HIV Antibodies/IMMUNOLOGY  HIV
       Antigens/BIOSYNTHESIS  HIV-1/IMMUNOLOGY/*PHYSIOLOGY  Support, Non-U.S.
       Gov't  *Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

