HELP-INTRACELLULAR CALCIUM MEASUREMENT			H_PROG	V 1.2

The menu items can be chosen by pressing the menu number, the associated
hot key or using the space bar or arrow key to move to the appropriate
item and pressing enter.

1) Start Measuring Calcium: Does just what it says. 
The program starts a timer when the measurement starts, this allows you 
to synchronise drug and stimulus addition. The program automatically stops 
after a preset time. Either the experimental time set with the program or
the time set manually on the Hitachi, whichever comes first. You can also
stop before either of these times using the Stop menu item. If the machine
is set to automatically print a run, the timer will idle while it does this. 

The program prompts you if you try to start a new measurement before 
saving the old one. 

2) Stop Measuring Calcium: If you want to stop before the preset 
ending time.

3) Save Data: Saves the data in File 0 (the active file) in the
Hitachi. There is currently no way to get the data "live" as it is 
measured.  The program prompts you for a file name, you can use any of 
the editing keys described below. [Enter] accepts the filename, [Tab] 
pops up a list of file names and [ESC] abandons the task. 

The program copies the data to temporary files, then creates 
a CSV (comma separated value) file. This is somewhat slow, but I can speed 
it up later. The data is written as Time, WL1, WL2 and Ratio.

The data is then graphed as the ratios, the scale is automatic 
between 0 and Rmax for the run (this may give some peculiar graphs)
The algorithm that handles artefacts can also use a little work. There 
is another problem in the display, it may go off scale due to a 
problem in the way BASIC handles integers.

4) Change Calcium parameters: sends you to the parameter menu (menu 3)
where you can manually set the Hitachi parameters for wavelength, 
experimental duration etc.

5) Clear timeout: Should something go wrong, and you have a data 
overflow, the F-2000 will timeout (and display timeout on the F-2000
screen), you have to use this command to clear it to receive further 
commands.

6) Display Graph of saved files: Graphs the Ratio values in the saved 
files. But ONLY ratio files (single wavelength files have the wrong format).
 
7) Change experimental length. Allows you set shorter run times than that
set manualy on the Hitachi.

EDITING file name input:

You can edit the default string in the input line, rather than retype
it. The following are the valid editing keys:

     INSERT         Toggles the insert mode and over-type mode.
     DELETE         Delete character at cursor.
     BACKSPACE      Destructive backspace.
     LEFT ARROW     Move the cursor to the left.
     RIGHT ARROW    Move the cursor to the right.
     HOME           Move the cursor to the beginning of the field.
     END            Move the cursor to the end of the field.
     CTL-HOME       Move cursor to the start and erase the field.
     CTL-END        Erase from the cursor to the end of the field.
     CTL-BkSpace    Erase from the cursor to the start of the field.

The program "remembers" the filename from run to run, allowing for easier
data entry.


