       Document 0883
 DOCN  M95A0883
 TI    Humoral immune responses to recombinant tree pollen allergens (Bet v I
       and Bet v II) in mice: construction of a live oral allergy vaccine.
 DT    9510
 AU    Vrtala S; Grote M; Ferreira F; Susani M; Stocker B; Kraft D; Valenta R;
       Institute of General and Experimental Pathology, AKH, University; of
       Vienna, Austria.
 SO    Int Arch Allergy Immunol. 1995 May-Jun;107(1-3):290-4. Unique Identifier
       : AIDSLINE MED/95337759
 AB    Recombinant tree pollen allergens (recombinant Bet v I and recombinant
       birch profilin, Bet v II) were purified and used to immunize BALB/c and
       B6D2F1 mice with Al(OH)3 to elicit a specific IgE response. Serum from
       immunized mice was then used to detect immunoblotted natural tree pollen
       allergens. The onset of the humoral immune response was monitored using
       antimouse IgE, IgG1, IgG2a/b, IgG3 and IgA. In both strains, a specific
       and long-lasting IgE response could be elicited with both recombinant
       allergens. Mice immunized continuously with recombinant Bet v I +
       Al(OH)3 showed a significant decrease of specific IgE antibodies
       indicating that continuous application of allergens can reduce specific
       IgE responses. The possibility of inducing a different type of immune
       responses is indicated by the fact that mice fed with Bet v I expressed
       in apathogenic Salmonella strains showed a Th1 immune response to Bet v
       I accompanied by specific IgG2a/b without detectable IgG1 or IgE.
       Recombinant allergens can hence be used to decrease or even modulate
       specific IgE responses in vivo.
 DE    Adjuvants, Immunologic  Administration, Oral  Allergens/ADMINISTRATION &
       DOSAGE/GENETICS/*IMMUNOLOGY/  THERAPEUTIC USE  Aluminum Hydroxide
       Animal  Antibodies, Bacterial/BIOSYNTHESIS  Bacterial
       Vaccines/*ADMINISTRATION & DOSAGE  *Desensitization, Immunologic  Hay
       Fever/THERAPY  Human  IgE/*BIOSYNTHESIS/IMMUNOLOGY
       IgG/*BIOSYNTHESIS/IMMUNOLOGY  Immunization  Mice  Mice, Inbred BALB C
       Microfilament Proteins/ADMINISTRATION & DOSAGE/GENETICS/
       *IMMUNOLOGY/THERAPEUTIC USE  Plant Proteins/ADMINISTRATION &
       DOSAGE/GENETICS/*IMMUNOLOGY/  THERAPEUTIC USE  Pollen/*IMMUNOLOGY
       Recombinant Fusion Proteins/IMMUNOLOGY  Salmonella
       typhimurium/*IMMUNOLOGY/PATHOGENICITY  Support, Non-U.S. Gov't  Th1
       Cells/IMMUNOLOGY  Trees  Vaccines, Attenuated/ADMINISTRATION &
       DOSAGE/THERAPEUTIC USE  Vaccines, Synthetic/ADMINISTRATION &
       DOSAGE/IMMUNOLOGY/  *THERAPEUTIC USE  Virulence  JOURNAL ARTICLE  REVIEW
       REVIEW, TUTORIAL

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

