       Document 0806
 DOCN  M95A0806
 TI    Immunohistochemical changes in sigmoid colon after allogeneic and
       autologous bone marrow transplantation.
 DT    9510
 AU    Forbes GM; Erber WN; Herrmann RP; Davies JM; Collins BJ; Department of
       Gastroenterology, Royal Perth Hospital, Western; Australia.
 SO    J Clin Pathol. 1995 Apr;48(4):308-13. Unique Identifier : AIDSLINE
       MED/95340890
 AB    AIM--To determine whether there are characteristic immunohistological
       changes in the colonic mucosa in acute graft versus host disease (GvHD).
       METHODS--Consecutive allogeneic (n = 11) and autologous (n = 11) bone
       marrow transplant recipients underwent endoscopic biopsy of sigmoid
       mucosa before transplant and on day 30 post-transplant.
       Immunohistochemical staining and quantitation of intraepithelial and
       lamina propria mononuclear cells were undertaken using a panel of
       monoclonal antibodies and a Streptavidin-biotin alkaline phosphatase
       staining technique. RESULTS--In the allogeneic group (nine of whom had
       clinical acute GvHD) there was a fivefold increase in lamina propria
       CD16+ mononuclear cells (3.1 +/- 4.3 to 17.0 +/- 12.2 per 100 lamina
       propria nucleated cells), compared with autologous transplant recipients
       in whom this rise was twofold (5.5 +/- 4.6 to 10.6 +/- 7.1 per 100
       lamina propria nucleated cells). The CD16+ mononuclear cells had
       morphological appearances of tissue macrophages, but in neither the
       allogeneic nor autologous groups was there an increase in total
       macrophage numbers (CD14+). In patients with acute GvHD the lamina
       propria CD4+:CD8+ lymphocyte ratio fell (1.97 +/- 1.12 to 1.07 +/-
       1.01), primarily because of a fall in the number of lamina propria CD4+
       lymphocytes. In both allogeneic and autologous groups there was a fall
       in intraepithelial lymphocyte numbers, but there was no change in CD19+
       (B cell), CD25+ (interleukin-2 receptor positive) or CD56+ (natural
       killer) cell numbers. CONCLUSION--Following bone marrow transplantation,
       there appears to be upregulation of lamina propria tissue macrophage
       CD16 (an Fc receptor for IgG), a change which is more noticeable after
       allogeneic transplantation and which may be related to the development
       of acute GvHD. In patients with acute GvHD there was a fall in the
       lamina propria CD4+:CD8+ lymphocyte ratio. If these changes are
       functionally important, they may have significant implications for
       understanding the pathogenesis of GvHD.
 DE    Acute Disease  Adult  Antigens, CD/ANALYSIS  Bone Marrow
       Transplantation/*IMMUNOLOGY/PATHOLOGY  CD4-CD8 Ratio  Female  Graft vs
       Host Disease/*IMMUNOLOGY/PATHOLOGY  Human  Immunoenzyme Techniques
       Intestinal Mucosa/IMMUNOLOGY  Leukocyte Count  Lymphocyte
       Subsets/PATHOLOGY  Male  Middle Age  Receptors, IgG/ANALYSIS
       Sigmoid/*IMMUNOLOGY/PATHOLOGY  Support, Non-U.S. Gov't  Transplantation,
       Autologous  Transplantation, Homologous  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

