       Document 0801
 DOCN  M95A0801
 TI    Malignant and normal T cells show random use of T-cell receptor alpha
       chain variable regions in patients with cutaneous T-cell lymphoma.
 DT    9510
 AU    Longley J; Tyrrell L; Lu SZ; Farrell J; Ding TG; Yan S; Sallee D; Heald
       P; Berger C; Tigelaar R; et al; Yale University School of Medicine,
       Department of Dermatology,; New Haven, CT 06520-8059, USA.
 SO    J Invest Dermatol. 1995 Jul;105(1):62-4. Unique Identifier : AIDSLINE
       MED/95341028
 AB    Cutaneous T-cell lymphoma (CTCL) is a malignancy of mature T
       lymphocytes, most of which express alpha/beta type T-cell receptors
       (TCRs). The cause of CTCL is unknown, but hypotheses postulating chronic
       stimulation of TCRs by superantigen or by a leukemogenic virus have been
       proposed. Either mechanism might produce bias in the TCR variable (V)
       region types used by the malignant cells. To determine if TCR alpha use
       is restricted in CTCL, we used reverse transcription and the polymerase
       chain reaction to determine V alpha and V beta usage by malignant cells
       purified from the peripheral blood of leukemic patients with CTCL. Usage
       of alpha chain V region segments appeared totally random; malignant
       lymphocytes isolated from each of six patients used different V alpha
       regions. As has been previously reported, no bias was found in beta
       chain V region usage either. In addition to productive (in frame) TCR V
       region mRNAs in malignant cells from each patient, we detected
       non-productive (out of frame) beta chain transcripts in these cells in
       two of six patients, and non-productive alpha chain transcripts in five
       of six. Residual normal peripheral blood lymphocytes from these patients
       showed a random, polyclonal or oligoclonal pattern of V region usage. We
       conclude that there is no bias in V region usage in CTCL, making it
       unlikely that interactions between superantigen or virus and the TCR V
       regions play a role in the pathogenesis of CTCL.
 DE    Aged  Aged, 80 and over  Base Sequence  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  Human  Lymphoma, T-Cell, Cutaneous/*IMMUNOLOGY
       Molecular Sequence Data  Polymerase Chain Reaction  Receptors, Antigen,
       T-Cell, alpha-beta/*GENETICS  RNA, Messenger/ANALYSIS  Support, U.S.
       Gov't, P.H.S.  T-Lymphocytes/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

