       Document 0737
 DOCN  M95A0737
 TI    FIV infection of macrophages: in vitro and in vivo inhibition by
       dideoxycytidine 5'-triphosphate.
 DT    9510
 AU    Magnani M; Rossi L; Fraternale A; Silvotti L; Quintavalla F; Piedimonte
       G; Matteucci D; Baldinotti F; Bendinelli M; Institute of Biochemistry,
       University of Urbino, Italy.
 SO    Vet Immunol Immunopathol. 1995 May;46(1-2):151-8. Unique Identifier :
       AIDSLINE MED/95343524
 AB    We have evaluated in vitro and in vivo whether it is possible to protect
       cat macrophages from feline immunodeficiency virus (FIV) infection by
       the administration of dideoxycytidine 5'-triphosphate (DDCTP). Since
       cell membranes are impermeable to phosphorylated drugs we have
       encapsulated DDCTP into autologous erythrocytes and modified erythrocyte
       membranes to target these drug-loaded cells to macrophages. DDCTP-loaded
       erythrocytes reduced FIV production by macrophages infected in vitro or
       obtained from naturally or experimentally infected cats. The same
       treatment protected the majority of peritoneal macrophages during a 7
       month experimental FIV infection and reduced the percentage of
       circulating lymphocytes stained with an anti-p24 antibody. These results
       suggest that the administration of nucleoside analogues in
       phosphorylated form is feasible and their targeting to macrophages
       reduces FIV infection in vitro and in vivo.
 DE    Animal  Antiviral Agents/ADMINISTRATION & DOSAGE/*PHARMACOLOGY  Cats
       Deoxycytosine Nucleotides/ADMINISTRATION & DOSAGE/*PHARMACOLOGY  Drug
       Carriers  Erythrocytes  Feline Acquired Immunodeficiency
       Syndrome/BLOOD/*DRUG THERAPY/  IMMUNOLOGY  Female  Immunodeficiency
       Virus, Feline/DRUG EFFECTS/*PHYSIOLOGY  Lymphocytes/VIROLOGY
       Macrophages/*VIROLOGY  Macrophages, Peritoneal/VIROLOGY  Male  Specific
       Pathogen-Free Organisms  Support, Non-U.S. Gov't  Virus Replication/DRUG
       EFFECTS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

