       Document 0788
 DOCN  M9550788
 TI    Increment of CD8S6F1 cells in synovial fluid from patients with
       rheumatoid arthritis.
 DT    9505
 AU    Ueki Y; Eguchi K; Miyake S; Nagataki S; Tominaga Y; Department of
       Internal Medicine, Sasebo Chuo Hospital, Japan.
 SO    Ann Rheum Dis. 1994 Dec;53(12):816-22. Unique Identifier : AIDSLINE
       MED/95168927
 AB    OBJECTIVE--To investigate the role of CD8 cell subsets in the
       pathogenesis of rheumatoid arthritis (RA) and the phenotypes of T cells
       adherent or non-adherent to the target cells (endothelial cells and
       synovial cells) pre-treated with IL-1 beta. METHODS--The expression of
       S6F1 on CD8 cells and that of an activation marker on CD8 cells and CD8
       cell subsets was evaluated in specimens of peripheral blood and synovial
       fluid obtained from 15 patients with RA and 10 with osteoarthritis (OA)
       using a two- or three-colour immunofluorescence method for analysis.
       RESULTS--The percentage of CD8S6F1 cells among CD8 cells in synovial
       fluid was significantly greater than that of peripheral blood. Synovial
       fluid from RA patients had a greater percentage of CD8S6F1 cells
       compared with either peripheral blood of matched patients or synovial
       fluid of OA patients. The percentage of CD8HLA-DR cells in synovial
       fluid was markedly greater than that in paired samples of peripheral
       blood in patients with RA. In the CD8S6F1 cells from both groups of
       patients, synovial fluid showed an increased percentage of HLA-DR cells
       compared with peripheral blood. Similar results were observed in CD8
       cells lacking S6F1 expression (CD8S6F1-) from both groups of patients.
       There was no significant difference in the percentage of HLA-DR cells
       between CD8S6F1 and CD8S6F1- cell populations in peripheral blood. In
       contrast with peripheral blood, in synovial fluid of RA patients the
       percentage of HLA-DR cells in the CD8S6F1 cell population was markedly
       greater than that in the CD8S6F1- population. However, the percentage of
       HLA-DR cells in both cell populations was similar in synovial fluid of
       OA patients. In both the endothelial and the synovial cell adhesion
       assays, the percentage of CD8S6F1 among CD8 cells and the mean
       fluorescence intensity of S6F1 antigen on CD8S6F1 cells were
       significantly greater in the adherent T cell population than that in the
       non-adherent T cell population. CONCLUSION--These results suggest that
       increased expression of S6F1 antigen and the increased percentage of
       HLA-DR cells on CD8 cells in synovial fluid may be responsible for the
       migration of these cells into inflamed synovial tissues, and for
       cellular interactions between these cells and synovial cells or the
       extracellular matrix.
 DE    Adult  Aged  Antigens, CD8/ANALYSIS  Antigens, Surface/IMMUNOLOGY
       Arthritis, Rheumatoid/BLOOD/*IMMUNOLOGY  Cell Adhesion/IMMUNOLOGY
       CD8-Positive T-Lymphocytes/*IMMUNOLOGY  Endothelium/CYTOLOGY  Female
       Flow Cytometry  Fluorescent Antibody Technique  Human  HLA-DR
       Antigens/IMMUNOLOGY  Interleukin-1/PHARMACOLOGY  Lymphocyte Count  Male
       Middle Age  Synovial Fluid/CYTOLOGY/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

