       Document 0780
 DOCN  M9550780
 TI    A full-coordinate model of the polymerase domain of HIV-I reverse
       transcriptase and its interaction with a nucleic acid substrate.
 DT    9505
 AU    Setlik RF; Meyer DJ; Shibata M; Roskwitalski R; Ornstein RL; Rein R;
       Department of Biophysics, Roswell Park Cancer Institute, Buffalo,; New
       York 14263.
 SO    J Biomol Struct Dyn. 1994 Aug;12(1):037-60. Unique Identifier : AIDSLINE
       MED/95151194
 AB    We present a full-coordinate model of residues 1-319 of the polymerase
       domain of HIV-I reverse transcriptase. This model was constructed from
       the x-ray crystallographic structure of Jacobo-Molina et al.
       (Jacobo-Molina et al., P.N.A.S. USA 90, 6320-6324 (1993)) which is
       currently available to the degree of C-coordinates. The backbone and
       side-chain atoms were constructed using the MAXSPROUT suite of programs
       (L. Holm and C. Sander, J. Mol. Biol. 218, 183-194 (1991)) and refined
       through molecular modeling. A seven base pair A-form dsDNA was
       positioned in the nucleic acid binding cleft to represent the
       template-primer complex. The orientation of the template-primer complex
       in the nucleic acid binding cleft was guided by the positions of
       phosphorus atoms in the crystal structure.
 DE    Amino Acid Sequence  DNA Polymerases/*CHEMISTRY
       HIV-1/*CHEMISTRY/ENZYMOLOGY  Magnesium/PHYSIOLOGY  *Models, Molecular
       Molecular Sequence Data  Nucleic Acids/*CHEMISTRY  Protein Structure,
       Tertiary  Reverse Transcriptase/*CHEMISTRY  Sequence Alignment
       Substrate Specificity  Support, U.S. Gov't, Non-P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

