       Document 0777
 DOCN  M9550777
 TI    Viro-immunological studies in acute HIV-1 infection.
 DT    9505
 AU    Roos MT; de Leeuw NA; Claessen FA; Huisman HG; Kootstra NA; Meyaard L;
       Schellekens PT; Schuitemaker H; Miedema F; Department of Clinical
       Viro-Immunology, Central Laboratory of the; Netherlands Red Cross Blood
       Transfusion Service, Amsterdam.
 SO    AIDS. 1994 Nov;8(11):1533-8. Unique Identifier : AIDSLINE MED/95151229
 AB    OBJECTIVE: To monitor a patient who presented with symptomatic HIV-1
       infection for virological and immunological parameters in relation to
       the clinical course. METHODS: Virological studies included determination
       of frequency of productively HIV-1-infected peripheral blood mononuclear
       cells (PBMC) and viral RNA load in plasma and p24 antigenaemia.
       Immunological studies included the analysis of T-cell subsets, the
       expression of activation markers, CD45RO and CD45RA antigens, the
       frequency of cells programmed for death, and T-cell function. RESULTS:
       During the first week post onset of primary HIV-1 infection symptoms
       high plasma titres of p24 and HIV-1 RNA were observed. The number of
       productively HIV-1-infected PBMC peaked, coinciding with CD4+ T
       lymphocytopaenia, during week 2 when clinical improvement started. CD8+
       T lymphocytosis was observed 10 days post onset of clinical symptoms,
       the expanded cell population being of the CD8+CD38+, CD8+CD27+ and
       CD8+CD28- phenotype. CD8+ T lymphocytosis was paralleled by a high
       percentage of cells undergoing programmed cell death on in vitro
       culture. In vitro T-cell function was severely depressed during the
       first 10 days post onset of clinical symptoms. Within about 3 weeks,
       following resolution of clinical symptoms, phytohaemagglutinin-induced
       proliferation was restored to normal levels while responses to the CD3
       monoclonal antibody only showed a partial restoration. During follow-up,
       concomitant with the rise of activated CD8+ T cells, p24 antigen levels
       and viral RNA load in serum as well as the number of HIV-producing PBMC
       steeply declined after 2 weeks. CONCLUSION: These findings demonstrate
       HIV-1-induced abnormalities during severe clinical symptoms of primary
       HIV-1 infection. The subsequent strong immune response, which is
       believed to be responsible for efficient control of viral replication,
       appears to precede clinical improvement.
 DE    Acquired Immunodeficiency Syndrome/BLOOD/*IMMUNOLOGY/*VIROLOGY  Adult
       Antigens, CD/BLOOD  Apoptosis  Case Report  CD4-Positive
       T-Lymphocytes/IMMUNOLOGY  CD8-Positive T-Lymphocytes/IMMUNOLOGY
       Homosexuality, Male  Human  HIV Antibodies/BLOOD  HIV Core Protein
       p24/BLOOD  HIV-1/*ISOLATION & PURIF  Immunologic Memory  Male  Reference
       Values  RNA, Viral/BLOOD  Sex Behavior  T-Lymphocyte Subsets/IMMUNOLOGY
       T-Lymphocytes/*IMMUNOLOGY/*VIROLOGY  Time Factors  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

