       Document 0728
 DOCN  M9550728
 TI    Detection of human immunodeficiency virus, hepatitis B virus, and
       hepatitis C virus in donor eyes using polymerase chain reaction.
 DT    9505
 AU    Shimazaki J; Tsubota K; Sawa M; Kinoshita S; Ohkura T; Honda M;
       Department of Ophthalmology, Tokyo Dental College, Chiba, Japan.
 SO    Br J Ophthalmol. 1994 Nov;78(11):859-62. Unique Identifier : AIDSLINE
       MED/95151706
 AB    Detection of human immunodeficiency virus (HIV), hepatitis B virus
       (HBV), and hepatitis C virus (HCV) in donor eyes was performed. DNAs
       were extracted from the uvea, and they were amplified using the
       polymerase chain reaction (PCR). Amplified viral DNAs were detected with
       liquid hybridisation and chemiluminescent assay in which no radioactive
       materials were used. This method was shown to have a sensitivity limit
       of fewer than 10 copies of HIV, making it much more sensitive than the
       current techniques employed in eye banks. The method was applied to 120
       donor eyes, including four from donors seropositive for HBV. The HBV
       gene was detected in one case in which the donor's blood had not been
       tested for HBV. HIV and HCV genes were not detected in any of the
       samples. The assay could be an effective screening test for the
       detection of these viruses in eye bank eyes.
 DE    Adolescence  Adult  Aged  Aged, 80 and over  Base Sequence  Child
       Child, Preschool  Cornea/*VIROLOGY  *Corneal Transplantation  DNA,
       Viral/ANALYSIS  Eye/TRANSPLANTATION  Hepatitis B Virus/*ISOLATION &
       PURIF  Hepatitis C Viruses/*ISOLATION & PURIF  Human  HIV/*ISOLATION &
       PURIF  Middle Age  Molecular Sequence Data  Polymerase Chain Reaction
       Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

