       Document 0097
 DOCN  M9460097
 TI    Factors affecting invasion of HT-29 and HEp-2 epithelial cells by
       organisms of the Mycobacterium avium complex.
 DT    9408
 AU    Bermudez LE; Young LS; Kuzell Institute for Arthritis and Infectious
       Diseases,; California Pacific Medical Center Research Institute, San;
       Francisco 94115.
 SO    Infect Immun. 1994 May;62(5):2021-6. Unique Identifier : AIDSLINE
       MED/94222576
 AB    Organisms of the Mycobacterium avium complex cause disseminated
       blood-borne infection in patients with AIDS, who acquire the infection
       mainly through the gastrointestinal tract. Prior to causing infection,
       M. avium must colonize and invade the intestinal mucosa. This study
       examined the ability of several serovars of the M. avium complex to bind
       to and invade the HT-29 intestinal mucosal cell line and the HEp-2
       laryngeal cell line. Logarithmic-phase M. avium was more efficient in
       binding and invasion than organisms in the stationary phase of growth.
       Bacteria incubated at 37 and 40 degrees C adhered to and invaded HT-29
       cells more efficiently than bacteria cultured at 30 degrees C. The
       ability of M. avium to invade HT-29 and HEp-2 cells was inhibited when
       the cells were incubated with cytochalasin B prior to exposure to the
       bacterium, suggesting active participation of the mammalian cell in the
       process of internalization. Two protein kinase inhibitors, staurosporine
       and H7, blocked invasion of M. avium, and a specific tyrosine protein
       kinase inhibitor, genistein, also blocked the internalization but not
       the binding of bacteria. The findings suggest that M. avium binds to a
       specific receptor(s) on the epithelial cells and uses the cytoskeleton
       of the mammalian cell to become internalized.
 DE    Alkaloids/PHARMACOLOGY  Cytochalasin B/PHARMACOLOGY
       Epithelium/MICROBIOLOGY  Human  Intestinal Mucosa/MICROBIOLOGY
       Larynx/MICROBIOLOGY  Mycobacterium avium Complex/GROWTH &
       DEVELOPMENT/*PATHOGENICITY  Signal Transduction  Support, U.S. Gov't,
       P.H.S.  Temperature  Tumor Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

