       Document 0875
 DOCN  M9460875
 TI    Accuracy of supplementary serologic testing for human T-lymphotropic
       virus types I and II in US blood donors. Retrovirus Epidemiology Donor
       Study.
 DT    9404
 AU    Busch MP; Laycock M; Kleinman SH; Wages JW Jr; Calabro M; Kaplan JE;
       Khabbaz RF; Hollingsworth CG; Irwin Memorial Blood Centers, San
       Francisco, CA 94118.
 SO    Blood. 1994 Feb 15;83(4):1143-8. Unique Identifier : AIDSLINE
       MED/94154278
 AB    Blood donations in the United States have been screened for antibody to
       human T-lymphotropic virus type I (HTLV-I) by HTLV-I enzyme immunoassay
       (EIA) since November 1988. Specimens repeatedly found to be reactive by
       EIA undergo confirmation by supplementary serologic tests. We assessed
       the accuracy of blood center testing of 994 HTLV-I EIA repeat-reactive
       specimens in five US blood centers between November 1988 and December
       1991. Of 410 confirmed HTLV-I/II donations, 407 (99.3%) were infected
       with HTLV-I/II, as determined by polymerase chain reaction (PCR) (403
       cases) and by repeat serologic testing (4 cases). The three
       false-positive results occurred in the first year of testing. Of 425
       HTLV-indeterminate specimens, 6 (1.4%) were found to be infected by PCR
       (5 with HTLV-II and 1 with HTLV-I). None of 159 confirmatory
       test-negative donations was PCR positive. Of HTLV-I/II-seropositive
       specimens, 80.2% to 95.4% could be typed as HTLV-I or HTLV-II by
       type-specific serologic assays. These results support recommendations
       that HTLV-I/II-seropositive donors should be advised that they are
       infected with HTLV-I, HTLV-II, or HTLV-I/II (depending on results of
       type-specific assays). HTLV-indeterminate donors should be advised that
       their results only rarely indicate HTLV infection. HTLV confirmatory
       test-negative donors should be reassured that they are not infected with
       HTLV-I or HTLV-II.
 DE    *Blood Donors  Blood Transfusion/*STANDARDS  Human  HTLV-I/ISOLATION &
       PURIF  HTLV-I Antibodies/*BLOOD  HTLV-I
       Infections/BLOOD/*DIAGNOSIS/PREVENTION & CONTROL  HTLV-II/ISOLATION &
       PURIF  HTLV-II Antibodies/*BLOOD  HTLV-II
       Infections/BLOOD/*DIAGNOSIS/PREVENTION & CONTROL  Polymerase Chain
       Reaction/METHODS  Support, U.S. Gov't, P.H.S.  United States  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).

